α-Synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells

Jee Young Sung; Hyun Jung Lee; Eun Il Jeong; Yohan Oh; Joongkyu Park; Kyung Sun Kang; Kwang Chul Chung

doi:10.1016/j.neulet.2007.02.025

α-Synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells

Jee Young Sung, Hyun Jung Lee, Eun Il Jeong, Yohan Oh, Joongkyu Park, Kyung Sun Kang, Kwang Chul Chung

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

α-Synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human α-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood. Gap junctional intercellular communication (GJIC) is thought to be essential for maintaining cellular homeostasis and growth control. In the present study, the effect of α-synuclein overexpression on GJIC in human dopaminergic neuroblastoma SH-SY5Y cells was investigated. Cells overexpressing wild-type α-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. GJIC was decreased in cells overexpressing α-synuclein. In addition, α-synuclein binds directly to connexin-32 (Cx32). As such, the post-translational modification of Cx32 was enhanced in cells overexpressing α-synuclein. These findings suggest that α-synuclein can modulate GJIC in a dopaminergic neuronal cell line through specific binding to Cx32.

Original language	English
Pages (from-to)	289-293
Number of pages	5
Journal	Neuroscience Letters
Volume	416
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2007.02.025
Publication status	Published - 2007 Apr 18

Bibliographical note

Funding Information:
The authors are grateful to M.M. Mouradian and S.S. Scherer for providing cells and plasmids. This study was supported by a grant (M103KV010011-06K2201-01110 to K.C.C.) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology of Republic of Korea, by a Basic Research Grant of Korea Science and Engineering Foundation (R01-2004-000-10673-0 to K.C.C.), and partly by grants (A050181 and A060440 to K.C.C.) from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea, and partly by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-005-E00017 to K.C.C).

All Science Journal Classification (ASJC) codes

Neuroscience(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.neulet.2007.02.025

Cite this

@article{2b42185cea8a4e9f84607b6d6dec60ae,

title = "α-Synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells",

abstract = "α-Synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human α-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood. Gap junctional intercellular communication (GJIC) is thought to be essential for maintaining cellular homeostasis and growth control. In the present study, the effect of α-synuclein overexpression on GJIC in human dopaminergic neuroblastoma SH-SY5Y cells was investigated. Cells overexpressing wild-type α-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. GJIC was decreased in cells overexpressing α-synuclein. In addition, α-synuclein binds directly to connexin-32 (Cx32). As such, the post-translational modification of Cx32 was enhanced in cells overexpressing α-synuclein. These findings suggest that α-synuclein can modulate GJIC in a dopaminergic neuronal cell line through specific binding to Cx32.",

author = "Sung, {Jee Young} and Lee, {Hyun Jung} and Jeong, {Eun Il} and Yohan Oh and Joongkyu Park and Kang, {Kyung Sun} and Chung, {Kwang Chul}",

note = "Funding Information: The authors are grateful to M.M. Mouradian and S.S. Scherer for providing cells and plasmids. This study was supported by a grant (M103KV010011-06K2201-01110 to K.C.C.) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology of Republic of Korea, by a Basic Research Grant of Korea Science and Engineering Foundation (R01-2004-000-10673-0 to K.C.C.), and partly by grants (A050181 and A060440 to K.C.C.) from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea, and partly by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-005-E00017 to K.C.C).",

year = "2007",

month = apr,

day = "18",

doi = "10.1016/j.neulet.2007.02.025",

language = "English",

volume = "416",

pages = "289--293",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - α-Synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells

AU - Sung, Jee Young

AU - Lee, Hyun Jung

AU - Jeong, Eun Il

AU - Oh, Yohan

AU - Park, Joongkyu

AU - Kang, Kyung Sun

AU - Chung, Kwang Chul

N1 - Funding Information: The authors are grateful to M.M. Mouradian and S.S. Scherer for providing cells and plasmids. This study was supported by a grant (M103KV010011-06K2201-01110 to K.C.C.) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology of Republic of Korea, by a Basic Research Grant of Korea Science and Engineering Foundation (R01-2004-000-10673-0 to K.C.C.), and partly by grants (A050181 and A060440 to K.C.C.) from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea, and partly by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-005-E00017 to K.C.C).

PY - 2007/4/18

Y1 - 2007/4/18

N2 - α-Synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human α-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood. Gap junctional intercellular communication (GJIC) is thought to be essential for maintaining cellular homeostasis and growth control. In the present study, the effect of α-synuclein overexpression on GJIC in human dopaminergic neuroblastoma SH-SY5Y cells was investigated. Cells overexpressing wild-type α-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. GJIC was decreased in cells overexpressing α-synuclein. In addition, α-synuclein binds directly to connexin-32 (Cx32). As such, the post-translational modification of Cx32 was enhanced in cells overexpressing α-synuclein. These findings suggest that α-synuclein can modulate GJIC in a dopaminergic neuronal cell line through specific binding to Cx32.

AB - α-Synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human α-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood. Gap junctional intercellular communication (GJIC) is thought to be essential for maintaining cellular homeostasis and growth control. In the present study, the effect of α-synuclein overexpression on GJIC in human dopaminergic neuroblastoma SH-SY5Y cells was investigated. Cells overexpressing wild-type α-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. GJIC was decreased in cells overexpressing α-synuclein. In addition, α-synuclein binds directly to connexin-32 (Cx32). As such, the post-translational modification of Cx32 was enhanced in cells overexpressing α-synuclein. These findings suggest that α-synuclein can modulate GJIC in a dopaminergic neuronal cell line through specific binding to Cx32.

UR - http://www.scopus.com/inward/record.url?scp=33947605407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947605407&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2007.02.025

DO - 10.1016/j.neulet.2007.02.025

M3 - Article

C2 - 17337120

AN - SCOPUS:33947605407

SN - 0304-3940

VL - 416

SP - 289

EP - 293

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

α-Synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this