TY - JOUR
T1 - Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme
AU - Li, Yang
AU - Bjorklund, Stefan
AU - Jiang, Yi Wei
AU - Kim, Young Joon
AU - Lane, William S.
AU - Stillman, David J.
AU - Kornberg, Roger D.
PY - 1995/11/21
Y1 - 1995/11/21
N2 - Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.
AB - Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.
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U2 - 10.1073/pnas.92.24.10864
DO - 10.1073/pnas.92.24.10864
M3 - Article
C2 - 7479899
AN - SCOPUS:0028826925
SN - 0027-8424
VL - 92
SP - 10864
EP - 10868
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 24
ER -