Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy

Tomoyuki Mano, Alexandre Albanese, Hans Ulrich Dodt, Ali Erturk, Viviana Gradinaru, Jennifer B. Treweek, Atsushi Miyawaki, Kwanghun Chung, Hiroki R. Ueda

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34 Citations (Scopus)


In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous MiniSymposium presented at the Society for Neuroscience’s 2018 annual meeting.

Original languageEnglish
Pages (from-to)9330-9337
Number of pages8
JournalJournal of Neuroscience
Issue number44
Publication statusPublished - 2018 Oct 31

Bibliographical note

Funding Information:
The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ertürk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Mu-rakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation andtheNIH(1-DP2-ES027992)forsupport;theGradinarulaboratorythanksSripriyaRavindraKumar,GerardM. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director’s Office and NSF Neuronex for support. The authors declare no competing financial interests. *T.M. and A.A. contributed equally to this work.

Publisher Copyright:
© 2018 the authors.

All Science Journal Classification (ASJC) codes

  • Medicine(all)


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