Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy

Tomoyuki Mano; Alexandre Albanese; Hans Ulrich Dodt; Ali Erturk; Viviana Gradinaru; Jennifer B. Treweek; Atsushi Miyawaki; Kwanghun Chung; Hiroki R. Ueda

doi:10.1523/JNEUROSCI.1677-18.2018

Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy

Tomoyuki Mano, Alexandre Albanese, Hans Ulrich Dodt, Ali Erturk, Viviana Gradinaru, Jennifer B. Treweek, Atsushi Miyawaki, Kwanghun Chung, Hiroki R. Ueda

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous MiniSymposium presented at the Society for Neuroscience’s 2018 annual meeting.

Original language	English
Pages (from-to)	9330-9337
Number of pages	8
Journal	Journal of Neuroscience
Volume	38
Issue number	44
DOIs	https://doi.org/10.1523/JNEUROSCI.1677-18.2018
Publication status	Published - 2018 Oct 31

Bibliographical note

Funding Information:
The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ertürk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Mu-rakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation andtheNIH(1-DP2-ES027992)forsupport;theGradinarulaboratorythanksSripriyaRavindraKumar,GerardM. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director’s Office and NSF Neuronex for support. The authors declare no competing financial interests. *T.M. and A.A. contributed equally to this work.

Publisher Copyright:
© 2018 the authors.

All Science Journal Classification (ASJC) codes

Medicine(all)

Access to Document

10.1523/JNEUROSCI.1677-18.2018

Cite this

@article{59828f38d16a469b8a85ad339c14ce6d,

title = "Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy",

abstract = "In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous MiniSymposium presented at the Society for Neuroscience{\textquoteright}s 2018 annual meeting.",

author = "Tomoyuki Mano and Alexandre Albanese and Dodt, {Hans Ulrich} and Ali Erturk and Viviana Gradinaru and Treweek, {Jennifer B.} and Atsushi Miyawaki and Kwanghun Chung and Ueda, {Hiroki R.}",

note = "Funding Information: The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ert{\"u}rk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Mu-rakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation andtheNIH(1-DP2-ES027992)forsupport;theGradinarulaboratorythanksSripriyaRavindraKumar,GerardM. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director{\textquoteright}s Office and NSF Neuronex for support. The authors declare no competing financial interests. *T.M. and A.A. contributed equally to this work. Publisher Copyright: {\textcopyright} 2018 the authors.",

year = "2018",

month = oct,

day = "31",

doi = "10.1523/JNEUROSCI.1677-18.2018",

language = "English",

volume = "38",

pages = "9330--9337",

journal = "Journal of Neuroscience",

issn = "0270-6474",

publisher = "Society for Neuroscience",

number = "44",

}

TY - JOUR

T1 - Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy

AU - Mano, Tomoyuki

AU - Albanese, Alexandre

AU - Dodt, Hans Ulrich

AU - Erturk, Ali

AU - Gradinaru, Viviana

AU - Treweek, Jennifer B.

AU - Miyawaki, Atsushi

AU - Chung, Kwanghun

AU - Ueda, Hiroki R.

N1 - Funding Information: The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ertürk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Mu-rakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation andtheNIH(1-DP2-ES027992)forsupport;theGradinarulaboratorythanksSripriyaRavindraKumar,GerardM. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director’s Office and NSF Neuronex for support. The authors declare no competing financial interests. *T.M. and A.A. contributed equally to this work. Publisher Copyright: © 2018 the authors.

PY - 2018/10/31

Y1 - 2018/10/31

N2 - In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous MiniSymposium presented at the Society for Neuroscience’s 2018 annual meeting.

AB - In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous MiniSymposium presented at the Society for Neuroscience’s 2018 annual meeting.

UR - http://www.scopus.com/inward/record.url?scp=85055776325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055776325&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.1677-18.2018

DO - 10.1523/JNEUROSCI.1677-18.2018

M3 - Article

C2 - 30381424

AN - SCOPUS:85055776325

SN - 0270-6474

VL - 38

SP - 9330

EP - 9337

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 44

ER -

Whole-brain analysis of cells and circuits by tissue clearing and light-sheet microscopy

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this