Whitening effect of adipose-derived stem cells: A critical role of TGF-β1

Won Serk Kim, So Hyun Park, Se Jin Ahn, Hyung Ki Kim, Jeong Soo Park, Ga Young Lee, Kea Jeung Kim, Kyu Kwang Whang, Seung Hee Kang, Byung Soon Park, Jong Hyuk Sung

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74 Citations (Scopus)


It has been demonstrated that adipose-derived stem cells (ADSCs) secrete cytokines and exhibit diverse pharmacological actions. The present study examined the unknown pharmacological action of ADSCs regarding whitening effects. A conditioned medium of ADSCs (ADSC-CM) was harvested and the whitening effect of ADSC-CM was studied in melanoma B16 cells. ADSC-CM treatment inhibited the synthesis of melanin and the activity of tyrosinase in a dose dependent manner. To clarify the underlying mechanisms of the whitening action of ADSCs, protein levels of melanogenic proteins were measured by Western blot. Although expressions of microphthalmia-associated transcription factor and tyrosinase-related protein 2 (TRP2) remained unchanged, those of tyrosinase and TRP1 were down-regulated. Transforming growth factor-β1 (TGF-β1), a potent regulator of melanogenic proteins, was neutralized by the addition of a blocking antibody to ADSC-CM, and down-regulated expression of tyrosinase and TRP1 was almost reversed. Collectively, these results indicate that secretary factors of ADSC inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1, which are mainly mediated by TGF-β1.

Original languageEnglish
Pages (from-to)606-610
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Issue number4
Publication statusPublished - 2008 Apr

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science


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