Volumetric reconstruction of Brownian motion of a micrometer-size bead in water

Yoon Sung Bae, Jong In Song, Dug Young Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


An effective volumetric measurement and reconstruction scheme for imaging three-dimensional phase objects based on off-axis digital holography is presented. We have experimentally demonstrated the feasibility of our method by observing the Brownian motion of a 3 μm diameter polystyrene bead in water. From a single off-axis hologram of a diffracted field by a sample, a series of transverse intensity images for various axial positions were numerically generated. The center position of the bead sample was pinpointed with a nanometer-scale resolution in 3D space and updated with a frame rate of 30 frames/s. Detailed procedures for elimination of virtual images in an off-axis hologram, reduction of background DC noise, and extraction of exact electric fields in an off-axis hologram are described as advantages of our proposed method over conventional in-line holographic 3D measurement schemes.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalOptics Communications
Publication statusPublished - 2013

Bibliographical note

Funding Information:
This work has been financially supported by the MEST through the National Research Foundation of Korea (Grant no. 012R1A4A102906 ) and by the Ministry of Education Science Technology of Korea for its financial support toward the Institute of Physics and Applied Physics at Yonsei University through BK21 program.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry
  • Electrical and Electronic Engineering


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