Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation

Juyoung Kwon; Jong Seung Lee; Jaejun Lee; Jukwan Na; Jaesuk Sung; Hyo Jung Lee; Hankyul Kwak; Eunji Cheong; Seung Woo Cho; Heon Jin Choi

doi:10.1021/acs.nanolett.0c04635

Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation

Juyoung Kwon, Jong Seung Lee, Jaejun Lee, Jukwan Na, Jaesuk Sung, Hyo Jung Lee, Hankyul Kwak, Eunji Cheong, Seung Woo Cho, Heon Jin Choi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.

Original language	English
Pages (from-to)	6343-6351
Number of pages	9
Journal	Nano letters
Volume	21
Issue number	14
DOIs	https://doi.org/10.1021/acs.nanolett.0c04635
Publication status	Published - 2021 Jul 28

Bibliographical note

Funding Information:
This research was supported by the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2018M3C7A1024654) and the Korean government (MSIP) (2017R1A2B3011586). This work was supported by a grant (2017M3C7A1047659) from the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (MSIT), Republic of Korea. This work was also supported by the Institute for Basic Science (IBS-R026-D1). This research was partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2019.

Publisher Copyright:
© 2021 American Chemical Society.

All Science Journal Classification (ASJC) codes

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.0c04635

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@article{c8ad7412fbf94e03a1aa03e8f108f449,

title = "Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation",

abstract = "Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.",

author = "Juyoung Kwon and Lee, {Jong Seung} and Jaejun Lee and Jukwan Na and Jaesuk Sung and Lee, {Hyo Jung} and Hankyul Kwak and Eunji Cheong and Cho, {Seung Woo} and Choi, {Heon Jin}",

note = "Funding Information: This research was supported by the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2018M3C7A1024654) and the Korean government (MSIP) (2017R1A2B3011586). This work was supported by a grant (2017M3C7A1047659) from the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (MSIT), Republic of Korea. This work was also supported by the Institute for Basic Science (IBS-R026-D1). This research was partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2019. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

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doi = "10.1021/acs.nanolett.0c04635",

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T1 - Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation

AU - Kwon, Juyoung

AU - Lee, Jong Seung

AU - Lee, Jaejun

AU - Na, Jukwan

AU - Sung, Jaesuk

AU - Lee, Hyo Jung

AU - Kwak, Hankyul

AU - Cheong, Eunji

AU - Cho, Seung Woo

AU - Choi, Heon Jin

N1 - Funding Information: This research was supported by the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2018M3C7A1024654) and the Korean government (MSIP) (2017R1A2B3011586). This work was supported by a grant (2017M3C7A1047659) from the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (MSIT), Republic of Korea. This work was also supported by the Institute for Basic Science (IBS-R026-D1). This research was partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2019. Publisher Copyright: © 2021 American Chemical Society.

PY - 2021/7/28

Y1 - 2021/7/28

N2 - Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.

AB - Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.

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Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation

Abstract

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