Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices

Seung Woo Cho, Hee Jung Park, Ju Hee Ryu, Soo Hyun Kim, Young Ha Kim, Cha Yong Choi, Min Jae Lee, Jong Sung Kim, In Sung Jang, Dong Ik Kim, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)


Synthetic polymer vascular patches used in cardiovascular surgery have shortcomings such as thrombosis, intimal hyperplasia, calcification, infection, and no growth potential. Tissue-engineered vascular patches using autologous vascular cells may solve these problems. In this study, we developed a tissue-engineered vascular patch using autologous bone marrow-derived cells (BMCs) and decellularized tissue matrices. Vascular smooth muscle cells and endothelial cells were differentiated from bone marrow mononuclear cells in vitro. Tissue-engineered vascular patches were fabricated by seeding these cells onto decellularized canine inferior vena cava matrices and implanted into the inferior vena cava of dogs. Three weeks after implantation, the tissue-engineered vascular patches were patent with no sign of thrombus formation. Histological, immunohistochemical, and electron microscopic analyses of the vascular patches retrieved 3 weeks after implantation revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular patches, indicating that the BMCs survived after implantation and contributed to the vascular tissue regeneration. This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and decellularized tissue matrices.

Original languageEnglish
Pages (from-to)1915-1924
Number of pages10
Issue number14
Publication statusPublished - 2005 May

Bibliographical note

Funding Information:
This work was supported by the Korea Health 21 R&D Project, the Ministry of Health and Welfare, Republic of Korea (02-PJ1-PG3-21104-0003).

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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