Valorization of leftover green tea residues through conversion to bioactive peptides using probiotics-aided anaerobic digestion

Ji Young Lee, Hyein Hong, Jae Eun Lee, Yi Jee Hong, Hye Won Hwang, Hyeon Su Jin, Hyunkyou Shim, Yong deog Hong, Won Seok Park, Jin Oh Chung, Dong Woo Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Bioactive peptides (BPs) are protein fragments that benefit human health. To assess whether leftover green tea residues (GTRs) can serve as a resource for new BPs, we performed in silico proteolysis of GTRs using the BIOPEP database, revealing a wide range of BPs embedded in GTRs. Comparative genomics and the percentage of conserved protein analyses enabled us to select a few probiotic strains for GTR hydrolysis. The selected probiotics digested GTRs anaerobically to yield GTR-derived peptide fractions. To examine whether green tea (GT) peptide fractions could be potential mediators of host–microbe interactions, we comprehensively screened agonistic and antagonistic activities of 168 human G protein-coupled receptors (GPCRs). NanoLC-MS/MS analysis and thin-layer chromatography allowed the identification of peptide sequences and the composition of glycan moieties in the GTRs. Remarkably, GT peptide fractions produced by Lactiplantibacillus plantarum APsulloc 331261, a strain isolated from GT, showed a potent-binding activity for P2RY6, a GPCR involved in intestinal homeostasis. Therefore, this study suggests the potential use of probiotics-aided GTR hydrolysates as postbiotic BPs, providing a biological process for recycling GTRs from agro-waste into renewable resources as health-promoting BPs.

Original languageEnglish
Pages (from-to)418-431
Number of pages14
JournalMicrobial Biotechnology
Issue number2
Publication statusPublished - 2023 Feb

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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