Using FLIM in the study of permeability barrier function of aged and young skin

P. Xu; E. H. Choi; M. Q. Man; D. Crumrine; T. Mauro; P. Elias

doi:10.1117/12.660985

Using FLIM in the study of permeability barrier function of aged and young skin

P. Xu
, E. H. Choi
, M. Q. Man
, D. Crumrine
, T. Mauro
, P. Elias

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing. Endogenous Na ⁺/H ⁺ antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
DOIs	https://doi.org/10.1117/12.660985
Publication status	Published - 2006
Event	Multiphoton Microscopy in the Biomedical Sciences VI - San Jose, CA, United States Duration: 2006 Jan 22 → 2006 Jan 24

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6089
ISSN (Print)	0277-786X

Other

Other	Multiphoton Microscopy in the Biomedical Sciences VI
Country/Territory	United States
City	San Jose, CA
Period	06/1/22 → 06/1/24

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.660985

Cite this

@inproceedings{4714aff2614140878b546cb93c615cff,

title = "Using FLIM in the study of permeability barrier function of aged and young skin",

abstract = "Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing. Endogenous Na +/H + antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.",

keywords = "BCECF, FLIM, Permeability barrier function, Stratum corneum, pH",

author = "P. Xu and Choi, \{E. H.\} and Man, \{M. Q.\} and D. Crumrine and T. Mauro and P. Elias",

year = "2006",

doi = "10.1117/12.660985",

language = "English",

isbn = "0819461318",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Multiphoton Microscopy in the Biomedical Sciences VI ; Conference date: 22-01-2006 Through 24-01-2006",

}

Xu, P, Choi, EH, Man, MQ, Crumrine, D, Mauro, T & Elias, P 2006, Using FLIM in the study of permeability barrier function of aged and young skin. in Proceedings of SPIE - The International Society for Optical Engineering., 60890Q, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6089, Multiphoton Microscopy in the Biomedical Sciences VI, San Jose, CA, United States, 06/1/22. https://doi.org/10.1117/12.660985

Using FLIM in the study of permeability barrier function of aged and young skin. / Xu, P.; Choi, E. H.; Man, M. Q. et al.
Proceedings of SPIE - The International Society for Optical Engineering. 2006. 60890Q (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6089).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Using FLIM in the study of permeability barrier function of aged and young skin

AU - Xu, P.

AU - Choi, E. H.

AU - Man, M. Q.

AU - Crumrine, D.

AU - Mauro, T.

AU - Elias, P.

PY - 2006

Y1 - 2006

N2 - Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing. Endogenous Na +/H + antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.

AB - Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing. Endogenous Na +/H + antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.

KW - BCECF

KW - FLIM

KW - Permeability barrier function

KW - Stratum corneum

KW - pH

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SN - 0819461318

SN - 9780819461315

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Multiphoton Microscopy in the Biomedical Sciences VI

Y2 - 22 January 2006 through 24 January 2006

ER -

Using FLIM in the study of permeability barrier function of aged and young skin

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