Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells

Hyun Kyu Kang; Ha Young Lee; Youl Nam Lee; Eun Jin Jo; Jung Im Kim; Gi Young Kim; Yeong Min Park; Do Sik Min; Akihiko Yano; Jong Young Kwak; Yoe Sik Bae

doi:10.1016/j.intimp.2004.04.001

Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells

Hyun Kyu Kang, Ha Young Lee, Youl Nam Lee, Eun Jin Jo, Jung Im Kim, Gi Young Kim, Yeong Min Park, Do Sik Min, Akihiko Yano, Jong Young Kwak, Yoe Sik Bae

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.

Original language	English
Pages (from-to)	911-920
Number of pages	10
Journal	International Immunopharmacology
Volume	4
Issue number	7
DOIs	https://doi.org/10.1016/j.intimp.2004.04.001
Publication status	Published - 2004 Jul

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation through the Medical Science and Engineering Research Center for Cancer Molecular Therapy at Dong-A University and by a grant 02-PJI-PG10-20706-0001 from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea.

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Pharmacology

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10.1016/j.intimp.2004.04.001

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@article{d73a166341e5442097d2040e92060a79,

title = "Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells",

abstract = "Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.",

author = "Kang, {Hyun Kyu} and Lee, {Ha Young} and Lee, {Youl Nam} and Jo, {Eun Jin} and Kim, {Jung Im} and Kim, {Gi Young} and Park, {Yeong Min} and Min, {Do Sik} and Akihiko Yano and Kwak, {Jong Young} and Bae, {Yoe Sik}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation through the Medical Science and Engineering Research Center for Cancer Molecular Therapy at Dong-A University and by a grant 02-PJI-PG10-20706-0001 from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea.",

year = "2004",

month = jul,

doi = "10.1016/j.intimp.2004.04.001",

language = "English",

volume = "4",

pages = "911--920",

journal = "International Immunopharmacology",

issn = "1567-5769",

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T1 - Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells

AU - Kang, Hyun Kyu

AU - Lee, Ha Young

AU - Lee, Youl Nam

AU - Jo, Eun Jin

AU - Kim, Jung Im

AU - Kim, Gi Young

AU - Park, Yeong Min

AU - Min, Do Sik

AU - Yano, Akihiko

AU - Kwak, Jong Young

AU - Bae, Yoe Sik

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation through the Medical Science and Engineering Research Center for Cancer Molecular Therapy at Dong-A University and by a grant 02-PJI-PG10-20706-0001 from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea.

PY - 2004/7

Y1 - 2004/7

N2 - Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.

AB - Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.

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JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 7

ER -

Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells

Abstract

Bibliographical note

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