UNC119a bridges the transmission of Fyn signals to RAB11, leading to the completion of cytokinesis

Yu Kyung Lee; Sunglan Chung; In Keol Baek; Tae H. Lee; Soon Young Paik; Joo Hun Lee

doi:10.4161/cc.24404

UNC119a bridges the transmission of Fyn signals to RAB11, leading to the completion of cytokinesis

Yu Kyung Lee, Sunglan Chung, In Keol Baek, Tae H. Lee, Soon Young Paik, Joo Hun Lee

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Src family kinases (SFKs) regulate the completion of cytokinesis through signal transduction pathways that lead to the Rab11-dependent phosphorylation of ERK and its localization to the midbody of cytokinetic cells. We find that UNC119a, a known activator of SFKs, plays essential roles in this signaling pathway. UNC119a localizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells. We show that the midbody localization of UNC119a is dependent on Rab11, and that knocking down UNC119a inhibits the Rab11-dependent phosphorylation and midbody localization of ERK and cytokinesis. Moreover, we demonstrate that UNC119a interacts with a Src family kinase, Fyn and is required for the activation of this kinase. These results suggest that UNC119a plays a key role in the Fyn signal transduction pathway, which regulates the completion of cytokinesis via Rab11.

Original language	English
Pages (from-to)	1303-1315
Number of pages	13
Journal	Cell Cycle
Volume	12
Issue number	8
DOIs	https://doi.org/10.4161/cc.24404
Publication status	Published - 2013 Apr 15

Bibliographical note

Funding Information:
Authors thank to Dr Jon Soderholm for proofreading this manuscript. We also thank to Waterborne Virus Bank (The Catholic University) for generous gift of materials. This work was supported by a grant from the National Research Foundation (NRF 2009-0079383, Mid-Career Researcher Program) funded by Ministry of Education, Science, and Technology, Republic of Korea.

All Science Journal Classification (ASJC) codes

Molecular Biology
Developmental Biology
Cell Biology

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title = "UNC119a bridges the transmission of Fyn signals to RAB11, leading to the completion of cytokinesis",

abstract = "Src family kinases (SFKs) regulate the completion of cytokinesis through signal transduction pathways that lead to the Rab11-dependent phosphorylation of ERK and its localization to the midbody of cytokinetic cells. We find that UNC119a, a known activator of SFKs, plays essential roles in this signaling pathway. UNC119a localizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells. We show that the midbody localization of UNC119a is dependent on Rab11, and that knocking down UNC119a inhibits the Rab11-dependent phosphorylation and midbody localization of ERK and cytokinesis. Moreover, we demonstrate that UNC119a interacts with a Src family kinase, Fyn and is required for the activation of this kinase. These results suggest that UNC119a plays a key role in the Fyn signal transduction pathway, which regulates the completion of cytokinesis via Rab11.",

author = "Lee, {Yu Kyung} and Sunglan Chung and Baek, {In Keol} and Lee, {Tae H.} and Paik, {Soon Young} and Lee, {Joo Hun}",

note = "Funding Information: Authors thank to Dr Jon Soderholm for proofreading this manuscript. We also thank to Waterborne Virus Bank (The Catholic University) for generous gift of materials. This work was supported by a grant from the National Research Foundation (NRF 2009-0079383, Mid-Career Researcher Program) funded by Ministry of Education, Science, and Technology, Republic of Korea.",

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AU - Lee, Yu Kyung

AU - Chung, Sunglan

AU - Baek, In Keol

AU - Lee, Tae H.

AU - Paik, Soon Young

AU - Lee, Joo Hun

N1 - Funding Information: Authors thank to Dr Jon Soderholm for proofreading this manuscript. We also thank to Waterborne Virus Bank (The Catholic University) for generous gift of materials. This work was supported by a grant from the National Research Foundation (NRF 2009-0079383, Mid-Career Researcher Program) funded by Ministry of Education, Science, and Technology, Republic of Korea.

PY - 2013/4/15

Y1 - 2013/4/15

N2 - Src family kinases (SFKs) regulate the completion of cytokinesis through signal transduction pathways that lead to the Rab11-dependent phosphorylation of ERK and its localization to the midbody of cytokinetic cells. We find that UNC119a, a known activator of SFKs, plays essential roles in this signaling pathway. UNC119a localizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells. We show that the midbody localization of UNC119a is dependent on Rab11, and that knocking down UNC119a inhibits the Rab11-dependent phosphorylation and midbody localization of ERK and cytokinesis. Moreover, we demonstrate that UNC119a interacts with a Src family kinase, Fyn and is required for the activation of this kinase. These results suggest that UNC119a plays a key role in the Fyn signal transduction pathway, which regulates the completion of cytokinesis via Rab11.

AB - Src family kinases (SFKs) regulate the completion of cytokinesis through signal transduction pathways that lead to the Rab11-dependent phosphorylation of ERK and its localization to the midbody of cytokinetic cells. We find that UNC119a, a known activator of SFKs, plays essential roles in this signaling pathway. UNC119a localizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells. We show that the midbody localization of UNC119a is dependent on Rab11, and that knocking down UNC119a inhibits the Rab11-dependent phosphorylation and midbody localization of ERK and cytokinesis. Moreover, we demonstrate that UNC119a interacts with a Src family kinase, Fyn and is required for the activation of this kinase. These results suggest that UNC119a plays a key role in the Fyn signal transduction pathway, which regulates the completion of cytokinesis via Rab11.

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UNC119a bridges the transmission of Fyn signals to RAB11, leading to the completion of cytokinesis

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