Ultrasonography of the internal architecture of the superficial part of the masseter muscle in vivo

Hyung Jin Lee, You Jin Choi, Kang Woo Lee, Kyung Seok Hu, Seong Taek Kim, Hee Jin Kim

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13 Citations (Scopus)


It is unclear whether the deep inferior tendon (DIT) is equally present in vivo, and little anatomical information is available regarding the existence and morphology of the DIT in healthy young subjects. The aim of this study was to characterize the DIT of the masseter muscle in healthy young subjects using ultrasonography and to compare the morphology of this tendon with previously reported data for healthy young subjects in order to provide the most-effective injection methods for botulinum neurotoxin treatments of masseteric hypertrophy. This study investigated two fresh cadavers and 30 healthy subjects. Ultrasonography scanning in both longitudinal and transverse directions was applied to the masseter muscle. The DIT within the superficial part of the masseter was observed in both the fresh cadavers and the living subjects. The posterior region of the masseter muscle was compartmentalized (entirely covered) by the DIT in 26.7% of the specimens. The superficial part of the masseter muscle was divided by the DIT transversely and longitudinally into the superficial and deep muscle bellies in 35% and 38.3% of the specimens, respectively. The present findings suggest applying a DIT-based injection technique under guidance by ultrasonography prior to treating masseteric hypertrophy. Clin. Anat. 32:446–452, 2019.

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalClinical Anatomy
Issue number3
Publication statusPublished - 2019 Apr

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (NRF-2017R1A2B4003781) and Daewoong Pharmaceutical Co., Ltd. The authors are grateful to the cadaver donors and their families who participated in the donation program. The authors thank Hwi Eun Hur (BA) from Davidson College for her assistance in revising the manuscript. The authors thank Su Hyun Chae (MS) from National Cancer Center for contributing illustrations to this manuscript.

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology


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