Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields

Kiheung Kim; Wonju Lee; Kyungwha Chung; Hongki Lee; Taehwang Son; Youngjin Oh; Yun Feng Xiao; Dong Ha Kim; Donghyun Kim

doi:10.1117/12.2291449

Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields

Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun Feng Xiao, Dong Ha Kim, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm² in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV
Editors	Dan V. Nicolau, Alexander N. Cartwright, Dror Fixler
Publisher	SPIE
ISBN (Electronic)	9781510614970
DOIs	https://doi.org/10.1117/12.2291449
Publication status	Published - 2018
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018 - San Francisco, United States Duration: 2018 Jan 30 → 2018 Jan 31

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10506
ISSN (Print)	1605-7422

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018
Country/Territory	United States
City	San Francisco
Period	18/1/30 → 18/1/31

Bibliographical note

Publisher Copyright:
© 2018 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2291449

Cite this

Kim, K., Lee, W., Chung, K., Lee, H., Son, T., Oh, Y., Xiao, Y. F., Kim, D. H., & Kim, D. (2018). Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. In D. V. Nicolau, A. N. Cartwright, & D. Fixler (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV Article 1050610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10506). SPIE. https://doi.org/10.1117/12.2291449

Kim, Kiheung ; Lee, Wonju ; Chung, Kyungwha et al. / Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV. editor / Dan V. Nicolau ; Alexander N. Cartwright ; Dror Fixler. SPIE, 2018. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields",

abstract = "The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.",

author = "Kiheung Kim and Wonju Lee and Kyungwha Chung and Hongki Lee and Taehwang Son and Youngjin Oh and Xiao, {Yun Feng} and Kim, {Dong Ha} and Donghyun Kim",

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year = "2018",

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language = "English",

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Kim, K, Lee, W, Chung, K, Lee, H, Son, T, Oh, Y, Xiao, YF, Kim, DH & Kim, D 2018, Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. in DV Nicolau, AN Cartwright & D Fixler (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV., 1050610, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10506, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018, San Francisco, United States, 18/1/30. https://doi.org/10.1117/12.2291449

Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. / Kim, Kiheung; Lee, Wonju; Chung, Kyungwha et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV. ed. / Dan V. Nicolau; Alexander N. Cartwright; Dror Fixler. SPIE, 2018. 1050610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10506).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Kim, Kiheung

AU - Lee, Wonju

AU - Chung, Kyungwha

AU - Lee, Hongki

AU - Son, Taehwang

AU - Oh, Youngjin

AU - Xiao, Yun Feng

AU - Kim, Dong Ha

AU - Kim, Donghyun

PY - 2018

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N2 - The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.

AB - The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.

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Kim K, Lee W, Chung K, Lee H, Son T, Oh Y et al. Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. In Nicolau DV, Cartwright AN, Fixler D, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV. SPIE. 2018. 1050610. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2291449

Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields

Abstract

Publication series

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Bibliographical note

All Science Journal Classification (ASJC) codes

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