Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Daesan Kim, Jaehyuk Lee, Boyoung Kim, Yujin Shin, Jinhong Park, Uijoo Kim, Minbaek Lee, Sang Bum Kim, Sunghoon Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

Original languageEnglish
Pages (from-to)640-649
Number of pages10
JournalNanoscale Advances
Volume5
Issue number3
DOIs
Publication statusPublished - 2022 Nov 22

Bibliographical note

Publisher Copyright:
© 2023 RSC.

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • General Chemistry
  • General Materials Science
  • General Engineering

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