Two-photon microscopy using an Yb3+-doped fiber laser with variable pulse widths

Dong Uk Kim, Hoseong Song, Woosub Song, Hyuk Sang Kwon, Miae Sung, Dug Young Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Most of the two-photon fluorescence microscopes are based on femtosecond Ti:Sapphire laser sources near the 800 nm wavelength. Here, we introduce a new confocal two-photon microscope system using a mode-locked Yb3+-doped fiber laser. The mode-locked fiber laser produces 13 ps pulses with large positive chirping at a repetition rate of 36 MHz with an average power of 80 mW. By using an external grating pair pulse compressor, the pulse width and the frequency chirping of the laser output are controlled for optimum two-photon excitation. For a given objective lens, the optimum condition was obtained by monitoring the two-photon-induced-photocurrent in a GaAsP photodiode at the sample position. The performance of this pulse width optimized two-photon microscope system was demonstrated by imaging Vybrant DiI-stained dorsal root ganglion cells in 2 and 3 dimensions.

Original languageEnglish
Pages (from-to)12341-12349
Number of pages9
JournalOptics Express
Issue number11
Publication statusPublished - 2012 May 21

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics


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