Two cyclic hexapeptides from Penicillium sp. FN070315 with antiangiogenic activities

Jun Pil Jang; Hye Jin Jung; Jang Mi Han; Narae Jung; Yonghyo Kim; Ho Jeong Kwon; Sung Kyun Ko; Nak Kyun Soung; Jae Hyuk Jang; Jong Seog Ahn

doi:10.1371/journal.pone.0184339

Two cyclic hexapeptides from Penicillium sp. FN070315 with antiangiogenic activities

Jun Pil Jang, Hye Jin Jung, Jang Mi Han, Narae Jung, Yonghyo Kim, Ho Jeong Kwon, Sung Kyun Ko, Nak Kyun Soung, Jae Hyuk Jang, Jong Seog Ahn

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8 Citations (Scopus)

Abstract

In the course of searching for angiogenesis inhibitors from microorganisms, two cyclic peptides, PF1171A (1) and PF1171C (2) were isolated from the soil fungus Penicillium sp. FN070315. In the present study, we investigated the antiangiogenic efficacy and associated mechanisms of 1 and 2 in vitro using human umbilical vein endothelial cells (HUVECs). Compounds 1 and 2 inhibited the proliferation of HUVECs at concentrations not exhibiting cytotoxicity. Moreover, 1 and 2 significantly suppressed vascular endothelial growth factor (VEGF)-induced migration, invasion, proliferation and tube formation of HUVECs as well as neovascularization of the chorioallantoic membrane in developing chick embryos. We also identified an association between the antiangiogenic activity of 1 and 2 and the downregulation of both the phosphorylation of VEGF receptor 2 and the expression of hypoxia inducible factor-1α at the protein level. Taken together, these results further suggest that compounds 1 and 2 will be promising angiogenesis inhibitors.

Original language	English
Article number	e0184339
Journal	PloS one
Volume	12
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0184339
Publication status	Published - 2017 Sept

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Publisher Copyright:
© 2017 Jang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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title = "Two cyclic hexapeptides from Penicillium sp. FN070315 with antiangiogenic activities",

abstract = "In the course of searching for angiogenesis inhibitors from microorganisms, two cyclic peptides, PF1171A (1) and PF1171C (2) were isolated from the soil fungus Penicillium sp. FN070315. In the present study, we investigated the antiangiogenic efficacy and associated mechanisms of 1 and 2 in vitro using human umbilical vein endothelial cells (HUVECs). Compounds 1 and 2 inhibited the proliferation of HUVECs at concentrations not exhibiting cytotoxicity. Moreover, 1 and 2 significantly suppressed vascular endothelial growth factor (VEGF)-induced migration, invasion, proliferation and tube formation of HUVECs as well as neovascularization of the chorioallantoic membrane in developing chick embryos. We also identified an association between the antiangiogenic activity of 1 and 2 and the downregulation of both the phosphorylation of VEGF receptor 2 and the expression of hypoxia inducible factor-1α at the protein level. Taken together, these results further suggest that compounds 1 and 2 will be promising angiogenesis inhibitors.",

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AU - Kim, Yonghyo

AU - Kwon, Ho Jeong

AU - Ko, Sung Kyun

AU - Soung, Nak Kyun

AU - Jang, Jae Hyuk

AU - Ahn, Jong Seog

N1 - Publisher Copyright: © 2017 Jang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2017/9

Y1 - 2017/9

N2 - In the course of searching for angiogenesis inhibitors from microorganisms, two cyclic peptides, PF1171A (1) and PF1171C (2) were isolated from the soil fungus Penicillium sp. FN070315. In the present study, we investigated the antiangiogenic efficacy and associated mechanisms of 1 and 2 in vitro using human umbilical vein endothelial cells (HUVECs). Compounds 1 and 2 inhibited the proliferation of HUVECs at concentrations not exhibiting cytotoxicity. Moreover, 1 and 2 significantly suppressed vascular endothelial growth factor (VEGF)-induced migration, invasion, proliferation and tube formation of HUVECs as well as neovascularization of the chorioallantoic membrane in developing chick embryos. We also identified an association between the antiangiogenic activity of 1 and 2 and the downregulation of both the phosphorylation of VEGF receptor 2 and the expression of hypoxia inducible factor-1α at the protein level. Taken together, these results further suggest that compounds 1 and 2 will be promising angiogenesis inhibitors.

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Two cyclic hexapeptides from Penicillium sp. FN070315 with antiangiogenic activities

Abstract

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