A new virus-like particle TTSV1 was isolated from the hyperthermophilic crenarchaeote Thermoproteus tenax sampled at a hot spring region in Indonesia. TTSV1 had a spherical shape with a diameter of approximately 70 nm and was morphologically similar to the PSV isolated from a strain of Pyrobaculum. The 21.6 kb linear double-stranded DNA genome of TTSV1 had 38 open reading frames (ORFs), of which 15 ORFs were most similar to those of PSV. The remaining 23 ORFs showed little similarity to proteins in the public databases. Southern blot analysis demonstrated that the viral genome is not integrated into the host chromosome. TTSV1 consisted of three putative structural proteins of 10, 20, and 35 kDa in size, and the 10-kDa major protein was identified by mass spectrometry as a TTSV1 gene product. TTSV1 could be assigned as a new member of the newly emerged Globuloviridae family that includes so far only one recently characterized virus PSV.
Bibliographical noteFunding Information:
We thank Dr. Maggy T. Suhartono and her co-workers at Inter University Center for Biotechnology, Bogor Agricultural University, Bogor, Indonesia, for their help in sampling hyperthermophiles from Indonesia thermal environments. This work was supported by the IMT 2000 Grant funded by the Korea Ministry of Commerce, Industry, and Energy (IMT 2000-00016108), and by the Korea Research Foundation Grant funded by the Korean Government (KRF-2004-C00148). LC-MS/MS analysis of TTSV1 viral proteins was supported by a grant (to K.P.K) from Biogreen 21 project in 2004.
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