Transient expression of phospholipase D1 in developing rat hippocampus

Do Sik Min; Jeong Sun Choi; Myung Hoon Chun; Jin Woong Chung; Mun Yong Lee

doi:10.1016/S0304-3940(01)02091-2

Transient expression of phospholipase D1 in developing rat hippocampus

Do Sik Min, Jeong Sun Choi, Myung Hoon Chun, Jin Woong Chung, Mun Yong Lee

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

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Abstract

We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

Original language	English
Pages (from-to)	125-128
Number of pages	4
Journal	Neuroscience Letters
Volume	310
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(01)02091-2
Publication status	Published - 2001 Sept 14

Bibliographical note

Funding Information:
The authors thank Mrs Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance. This work was supported by The Basic Research Program of the Korea Science & Engineering Foundation (Grant number: 2000-0-213-001-2).

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/S0304-3940(01)02091-2

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title = "Transient expression of phospholipase D1 in developing rat hippocampus",

abstract = "We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.",

author = "Min, {Do Sik} and Choi, {Jeong Sun} and Chun, {Myung Hoon} and Chung, {Jin Woong} and Lee, {Mun Yong}",

note = "Funding Information: The authors thank Mrs Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance. This work was supported by The Basic Research Program of the Korea Science & Engineering Foundation (Grant number: 2000-0-213-001-2).",

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AU - Choi, Jeong Sun

AU - Chun, Myung Hoon

AU - Chung, Jin Woong

AU - Lee, Mun Yong

N1 - Funding Information: The authors thank Mrs Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance. This work was supported by The Basic Research Program of the Korea Science & Engineering Foundation (Grant number: 2000-0-213-001-2).

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N2 - We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

AB - We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

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Transient expression of phospholipase D1 in developing rat hippocampus

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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