Tissue surface as the reference arm in Fourier domain optical coherence tomography

Nikola Krstajić, Christian Tom A. Brown, Kishan Dholakia, Mario Ettore Giardini

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


We present a simple method applicable to common-path Fourier domain optical coherence tomography (OCT) in which the tissue surface is used as the reference arm. We propose using aluminium hydroxide powder as a potential tissue surface diffuser to allow wider application of this method. This technique allows one to avoid placing a reference arm reflective element, such as glass plate, on tissue, and intrinsically avoids both coherent and complex conjugate mirror artifacts associated with glass plates. Aluminium hydroxide can be sprayed onto tissue using spray nozzles commonly found in endoscopes. The sensitivity of the tissue reference arm common-path OCT image is 94 dB for a 50-μs charge-coupled device integration time, and 97.5 dB for a 200-μs CCD integration time.

Original languageEnglish
Article number071305
JournalJournal of Biomedical Optics
Issue number7
Publication statusPublished - 2012 Jul

Bibliographical note

Funding Information:
This work has been funded by European Union FP7 project ARAKNES (www.araknes.org) under Grant Agreement No. 224565. Kishan Dholakia is a Royal Society Wolfson Research Merit Award holder. Intellectual property rights on this work are covered by the University of St. Andrews. Nikola Krstajić(NK) wishes to thank Cathy Shields for reviewing the manuscript. Significant sections of OCT software were written while NK was employed by the University of Sheffield. NK wishes to thank Dr. Steve Matcher and his group at the University of Sheffield for all their help in learning OCT techniques.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials


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