Tissue engineering scaffolds for controlled protein and plasmid delivery

L. D. Shea, K. J. Whittlesey, T. Segura, J. Jang, Y. Yang

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)


Tissue engineering approaches employ polymer scaffolds to supports, promote, and direct tissue formation by progenitor cells, which are either present in the surrounding tissue or transplanted with the polymer. We hypothesize that scaffolds capable of delivering tissue-inductive factors, or genes encoding for these factors, at the correct time and location, and in the appropriate concentration could enhance the effectiveness of current regenerative approaches. Scaffolds have been developed that provide a controlled, sustained delivery of protein and non-viral DNA. Polymeric scaffolds capable of sustained drug release can be fabricated by the assembly of drug-loaded microspheres and subsequent fusion of the microspheres using a gas foaming process. This process has been developed to regulate the amount released and the release rates for protein, naked DNA, and DNA complexed with cationic lipid and polymers. Alternatively, we have developed a controlled DNA delivery system based on the tethering of DNA complexes to a biomaterial surfaces. Complexes can be immobilized at high densities and cells cultured on the substrates can internalize and express the DNA. These systems for the controlled delivery of proteins, or DNA-encoding for these proteins, are versatile and can be readily applied to the regeneration of various tissues.

Original languageEnglish
Pages (from-to)474
Number of pages1
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Publication statusPublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: 2002 Oct 232002 Oct 26

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics


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