TiO₂ Nanowires from Wet-Corrosion Synthesis for Peptide Sequencing Using Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

In this work, TiO₂ nanowires synthesized from a wet-corrosion process were presented for peptide sequencing by photocatalytic reaction with UV radiation. For the photocatalytic decomposition of peptides, the peptide sample was dropped on a target plate containing synthesized TiO₂ nanowire zones and UV-irradiated. Subsequently, the target plate was analyzed by laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry using the synthesized TiO₂ nanowires as a solid matrix. The feasibility of peptide sequencing based on the photocatalytic reaction with the synthesized TiO₂ nanowires was demonstrated using six types of peptides GHP9 (G₁-H-P-Q-G₂-K₁-K₂-K₃-K₄, 1006.59 Da), BPA-1(K₁-S₁-L-E-N-S₂-Y-G₁-G₂-G₃-K₂-K₃-K₄, 1394.74 Da), PreS1(F₁-G-A-N₁-S-N₂-N₃-P₁-D₁-W-D₂-F₂-N₄-P₂-N₅, 1707.68 Da), HPQ peptide-1 (G-Y-H-P-Q-R-K, 884.45 Da), HPQ peptide-2 (K-R-H-P-Q-Y-G, 884.45 Da), and HPQ peptide-3 (R-Y-H-P-Q-G-K, 884.45 Da). The identification of three different peptides with the same molecular weight was also demonstrated by using the synthesized TiO₂ nanowires for their photocatalytic decomposition as well as for LDI-TOF mass spectrometry as a solid-matrix.