Thy-1 knockdown retards wound repair in mouse skin

Min Jung Lee; Jeong Oh Shin; Han Sung Jung

doi:10.1016/j.jdermsci.2012.11.009

Thy-1 knockdown retards wound repair in mouse skin

Min Jung Lee, Jeong Oh Shin, Han Sung Jung

Department of Oral Biology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Background: Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. Objective: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. Methods and results: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. Conclusion: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.

Original language	English
Pages (from-to)	95-104
Number of pages	10
Journal	Journal of Dermatological Science
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/j.jdermsci.2012.11.009
Publication status	Published - 2013 Feb

Bibliographical note

Funding Information:
We are grateful to Prof. Sung-Won Cho for critical reading of this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0015661).

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Dermatology

Access to Document

10.1016/j.jdermsci.2012.11.009

Cite this

@article{cc8f7b926f624becaa86535ccd63865b,

title = "Thy-1 knockdown retards wound repair in mouse skin",

abstract = "Background: Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. Objective: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. Methods and results: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. Conclusion: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.",

author = "Lee, {Min Jung} and Shin, {Jeong Oh} and Jung, {Han Sung}",

note = "Funding Information: We are grateful to Prof. Sung-Won Cho for critical reading of this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0015661).",

year = "2013",

month = feb,

doi = "10.1016/j.jdermsci.2012.11.009",

language = "English",

volume = "69",

pages = "95--104",

journal = "Journal of Dermatological Science",

issn = "0923-1811",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Thy-1 knockdown retards wound repair in mouse skin

AU - Lee, Min Jung

AU - Shin, Jeong Oh

AU - Jung, Han Sung

N1 - Funding Information: We are grateful to Prof. Sung-Won Cho for critical reading of this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0015661).

PY - 2013/2

Y1 - 2013/2

N2 - Background: Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. Objective: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. Methods and results: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. Conclusion: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.

AB - Background: Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. Objective: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. Methods and results: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. Conclusion: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.

UR - http://www.scopus.com/inward/record.url?scp=84873568553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873568553&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2012.11.009

DO - 10.1016/j.jdermsci.2012.11.009

M3 - Article

C2 - 23312577

AN - SCOPUS:84873568553

SN - 0923-1811

VL - 69

SP - 95

EP - 104

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

IS - 2

ER -

Thy-1 knockdown retards wound repair in mouse skin

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this