Three-dimensional visualization and quantitative analysis of cervical cell nuclei with confocal laser scanning microscopy

Hyun Ju Choi, Ik Hwan Choi, Tae Yun Kim, Nam Hoon Cho, Heung Kook Choi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


OBJECTIVE: To develop a method for the acquisition and processing of 3-dimensional images based on confocal laser scanning microscopy for the purpose of 3-dimensional visualization and quantitative analysis of cell nuclei. STUDY DESIGN: A contour-based surface rendering method was used, and volume rendering was implemented according to the basic volume rendering pipeline. To extract quantitative features, a 3-dimensional labeling method based on slice information was used. After applying the labeling algorithm, the measurements for 3-dimensional quantitative analysis of nuclei were extracted: nuclear volume, surface area and spherical shape factor. We compared the 3-dimensional features of normal and abnormal cervical cell nuclei. RESULTS: Comparison of the size of 3-dimensional cervical cell nuclei between normal and abnormal revealed a statistically significant difference. The proposed method could overcome the limitation inherent in 2-dimensional analysis and could become a way of improving the accuracy and reproducibility of quantification of cell nuclei. CONCLUSION: Three-dimensional visualization and quantification of cell nuclei provide valuable medical information that can lead to a more objective diagnosis.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Issue number3
Publication statusPublished - 2005 Jun

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology


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