Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes

Wonju Lee; Yoshiaki Kinosita; Youngjin Oh; Nagisa Mikami; Heejin Yang; Makoto Miyata; Takayuki Nishizaka; Donghyun Kim

doi:10.1021/acsnano.5b03934

Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes

Wonju Lee, Yoshiaki Kinosita, Youngjin Oh, Nagisa Mikami, Heejin Yang, Makoto Miyata, Takayuki Nishizaka, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding Mycoplasma mobile (M. mobile). The results analyzed with multiple M. mobile bacteria show individual characters and reveal that M. mobile undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision.

Original language	English
Pages (from-to)	10896-10908
Number of pages	13
Journal	ACS Nano
Volume	9
Issue number	11
DOIs	https://doi.org/10.1021/acsnano.5b03934
Publication status	Published - 2015 Nov 24

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

All Science Journal Classification (ASJC) codes

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/acsnano.5b03934

Cite this

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title = "Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes",

abstract = "In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding Mycoplasma mobile (M. mobile). The results analyzed with multiple M. mobile bacteria show individual characters and reveal that M. mobile undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision.",

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T1 - Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes

AU - Lee, Wonju

AU - Kinosita, Yoshiaki

AU - Oh, Youngjin

AU - Mikami, Nagisa

AU - Yang, Heejin

AU - Miyata, Makoto

AU - Nishizaka, Takayuki

AU - Kim, Donghyun

PY - 2015/11/24

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Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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