Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

Sewoon Han; Kisuk Yang; Yoojin Shin; Jung Seung Lee; Roger D. Kamm; Seok Chung; Seung Woo Cho

doi:10.1039/c2lc21285d

Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

Sewoon Han, Kisuk Yang, Yoojin Shin, Jung Seung Lee, Roger D. Kamm, Seok Chung, Seung Woo Cho

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.

Original language	English
Pages (from-to)	2305-2308
Number of pages	4
Journal	Lab on a chip
Volume	12
Issue number	13
DOIs	https://doi.org/10.1039/c2lc21285d
Publication status	Published - 2012 Jul 7

All Science Journal Classification (ASJC) codes

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

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AU - Yang, Kisuk

AU - Shin, Yoojin

AU - Lee, Jung Seung

AU - Kamm, Roger D.

AU - Chung, Seok

AU - Cho, Seung Woo

PY - 2012/7/7

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AB - Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.

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Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

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