Three-dimensional Cardiomyocytes Structure Revealed by Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique

Sang Eun Lee; Christopher Nguyen; Jongjin Yoon; Hyuk Jae Chang; Sekeun Kim; Chul Hoon Kim; Debiao Li

doi:10.1038/s41598-018-24622-6

Three-dimensional Cardiomyocytes Structure Revealed by Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique

Sang Eun Lee, Christopher Nguyen, Jongjin Yoon, Hyuk Jae Chang, Sekeun Kim, Chul Hoon Kim, Debiao Li

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n = 8) heart failure model mice. Whole heart fiber tracking using DTI in fixed ex-vivo mouse hearts was performed, and the hearts were processed with the tissue-clearing technique. Cardiomyocytes orientation was quantified on both DTI and 3D histology. Helix angle (HA) and global HA transmurality (HAT) were calculated, and the DTI findings were confirmed with 3D histology. Global HAT was significantly reduced in the ischemic group (DTI: 0.79 ± 0.13°/% transmural depth [TD] and 3D histology: 0.84 ± 0.26°/%TD) compared with controls (DTI: 1.31 ± 0.20°/%TD and 3D histology: 1.36 ± 0.27°/%TD, all p < 0.001). On direct comparison of DTI with 3D histology for the quantitative assessment of cardiomyocytes orientation, significant correlations were observed in both per-sample (R² = 0.803) and per-segment analyses (R² = 0.872). We demonstrated the capability and accuracy of DTI for mapping cardiomyocytes orientation by comparison with the intact 3D histology acquired by tissue-clearing technique. DTI is a promising tool for the noninvasive characterization of cardiomyocytes architecture.

Original language	English
Article number	6640
Journal	Scientific reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-24622-6
Publication status	Published - 2018 Dec 1

Bibliographical note

Funding Information:
Competing Interests: Dr. Chang receives funding from by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) (Grant No. 2012027176); This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (2012027176). For the remaining authors none were declared.

Funding Information:
Light-sheet fluorescence microscope (Lightsheet Z.1, Carl Zeiss Microscopy Co, Ltd. Germany) data were acquired at the Advanced Neural Imaging Center of the Korea Brain Research Institute, Daegu, Korea. The authors would like to thank Dong-Su Jang, MFA, (Medical Illustrator, Medical Research Support Section, Yonsei University College of Medicine, Seoul, Korea) for his help with the illustrations

Publisher Copyright:
© 2018 The Author(s).

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title = "Three-dimensional Cardiomyocytes Structure Revealed by Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique",

abstract = "We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n = 8) heart failure model mice. Whole heart fiber tracking using DTI in fixed ex-vivo mouse hearts was performed, and the hearts were processed with the tissue-clearing technique. Cardiomyocytes orientation was quantified on both DTI and 3D histology. Helix angle (HA) and global HA transmurality (HAT) were calculated, and the DTI findings were confirmed with 3D histology. Global HAT was significantly reduced in the ischemic group (DTI: 0.79 ± 0.13°/% transmural depth [TD] and 3D histology: 0.84 ± 0.26°/%TD) compared with controls (DTI: 1.31 ± 0.20°/%TD and 3D histology: 1.36 ± 0.27°/%TD, all p < 0.001). On direct comparison of DTI with 3D histology for the quantitative assessment of cardiomyocytes orientation, significant correlations were observed in both per-sample (R2 = 0.803) and per-segment analyses (R2 = 0.872). We demonstrated the capability and accuracy of DTI for mapping cardiomyocytes orientation by comparison with the intact 3D histology acquired by tissue-clearing technique. DTI is a promising tool for the noninvasive characterization of cardiomyocytes architecture.",

author = "Lee, {Sang Eun} and Christopher Nguyen and Jongjin Yoon and Chang, {Hyuk Jae} and Sekeun Kim and Kim, {Chul Hoon} and Debiao Li",

note = "Funding Information: Competing Interests: Dr. Chang receives funding from by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) (Grant No. 2012027176); This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (2012027176). For the remaining authors none were declared. Funding Information: Light-sheet fluorescence microscope (Lightsheet Z.1, Carl Zeiss Microscopy Co, Ltd. Germany) data were acquired at the Advanced Neural Imaging Center of the Korea Brain Research Institute, Daegu, Korea. The authors would like to thank Dong-Su Jang, MFA, (Medical Illustrator, Medical Research Support Section, Yonsei University College of Medicine, Seoul, Korea) for his help with the illustrations Publisher Copyright: {\textcopyright} 2018 The Author(s).",

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AU - Kim, Sekeun

AU - Kim, Chul Hoon

AU - Li, Debiao

N1 - Funding Information: Competing Interests: Dr. Chang receives funding from by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) (Grant No. 2012027176); This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (2012027176). For the remaining authors none were declared. Funding Information: Light-sheet fluorescence microscope (Lightsheet Z.1, Carl Zeiss Microscopy Co, Ltd. Germany) data were acquired at the Advanced Neural Imaging Center of the Korea Brain Research Institute, Daegu, Korea. The authors would like to thank Dong-Su Jang, MFA, (Medical Illustrator, Medical Research Support Section, Yonsei University College of Medicine, Seoul, Korea) for his help with the illustrations Publisher Copyright: © 2018 The Author(s).

PY - 2018/12/1

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N2 - We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n = 8) heart failure model mice. Whole heart fiber tracking using DTI in fixed ex-vivo mouse hearts was performed, and the hearts were processed with the tissue-clearing technique. Cardiomyocytes orientation was quantified on both DTI and 3D histology. Helix angle (HA) and global HA transmurality (HAT) were calculated, and the DTI findings were confirmed with 3D histology. Global HAT was significantly reduced in the ischemic group (DTI: 0.79 ± 0.13°/% transmural depth [TD] and 3D histology: 0.84 ± 0.26°/%TD) compared with controls (DTI: 1.31 ± 0.20°/%TD and 3D histology: 1.36 ± 0.27°/%TD, all p < 0.001). On direct comparison of DTI with 3D histology for the quantitative assessment of cardiomyocytes orientation, significant correlations were observed in both per-sample (R2 = 0.803) and per-segment analyses (R2 = 0.872). We demonstrated the capability and accuracy of DTI for mapping cardiomyocytes orientation by comparison with the intact 3D histology acquired by tissue-clearing technique. DTI is a promising tool for the noninvasive characterization of cardiomyocytes architecture.

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Three-dimensional Cardiomyocytes Structure Revealed by Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique

Abstract

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