Thermophoretic immunoassay based on autodisplayed Z-domains for the diagnosis of C-reactive protein

Ga Yeon Lee; Park Min Park; Min Jung Kang; Klaus Langer; Joachim Jose; Jae Chul Pyun

doi:10.1016/j.snb.2017.12.019

Thermophoretic immunoassay based on autodisplayed Z-domains for the diagnosis of C-reactive protein

Ga Yeon Lee, Park Min Park, Min Jung Kang, Klaus Langer, Joachim Jose, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Immunoassays are used to detect a target analyte by using specific interaction with detection antibodies which form antigen-antibody complexes. In thermophoretic immunoassays the antigen-antibody complexes make slow thermophoresis (movement by thermal stimulation) in comparison with unbound antigens and antibodies. In this work, a sensitive thermophoretic immunoassay was demonstrated by mixing outer membrane (OM) particles of E. coli with autodisplayed Z-domains. In order to compare the influence of OM particles with autodisplayed Z-domains on the sensitivity of thermophoretic immunoassay, the conventional method only with target analyte and detection antibodies was compared with the hyper sensitive thermophoretic immunoassay by mixing OM particles with autodisplayed Z-domains, and the affinity constant was calculated. Finally, the diagnosis of inflammation by detection of a biomarker called C-reactive protein (CRP) was carried out by demonstrating the thermophoretic immunoassay for CRP based on autodisplayedZ-domains could be applied for the medical diagnosis.

Original language	English
Pages (from-to)	1131-1137
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	258
DOIs	https://doi.org/10.1016/j.snb.2017.12.019
Publication status	Published - 2018 Apr 1

Bibliographical note

Funding Information:
We are very grateful to K. Langer, Pharm. Tech., WWU Münster for the opportunity to do the photon correlation spectroscopy experiments in his lab. This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the National Research Foundation of Korea [grant number: NRF-2017R1A6A3A11034081, NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2016R1D1A3B03934169 ], and by the Yonsei University Research Fund(Yonsei Frontier Lab. Young Researcher Supporting Program) of 2017, and by the Hallym University Research Fund , 2017 (HRF-201709-014).

Publisher Copyright:
© 2017 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2017.12.019

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title = "Thermophoretic immunoassay based on autodisplayed Z-domains for the diagnosis of C-reactive protein",

abstract = "Immunoassays are used to detect a target analyte by using specific interaction with detection antibodies which form antigen-antibody complexes. In thermophoretic immunoassays the antigen-antibody complexes make slow thermophoresis (movement by thermal stimulation) in comparison with unbound antigens and antibodies. In this work, a sensitive thermophoretic immunoassay was demonstrated by mixing outer membrane (OM) particles of E. coli with autodisplayed Z-domains. In order to compare the influence of OM particles with autodisplayed Z-domains on the sensitivity of thermophoretic immunoassay, the conventional method only with target analyte and detection antibodies was compared with the hyper sensitive thermophoretic immunoassay by mixing OM particles with autodisplayed Z-domains, and the affinity constant was calculated. Finally, the diagnosis of inflammation by detection of a biomarker called C-reactive protein (CRP) was carried out by demonstrating the thermophoretic immunoassay for CRP based on autodisplayedZ-domains could be applied for the medical diagnosis.",

author = "Lee, {Ga Yeon} and {Min Park}, Park and Kang, {Min Jung} and Klaus Langer and Joachim Jose and Pyun, {Jae Chul}",

note = "Funding Information: We are very grateful to K. Langer, Pharm. Tech., WWU M{\"u}nster for the opportunity to do the photon correlation spectroscopy experiments in his lab. This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the National Research Foundation of Korea [grant number: NRF-2017R1A6A3A11034081, NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2016R1D1A3B03934169 ], and by the Yonsei University Research Fund(Yonsei Frontier Lab. Young Researcher Supporting Program) of 2017, and by the Hallym University Research Fund , 2017 (HRF-201709-014). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

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AU - Jose, Joachim

AU - Pyun, Jae Chul

N1 - Funding Information: We are very grateful to K. Langer, Pharm. Tech., WWU Münster for the opportunity to do the photon correlation spectroscopy experiments in his lab. This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the National Research Foundation of Korea [grant number: NRF-2017R1A6A3A11034081, NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2016R1D1A3B03934169 ], and by the Yonsei University Research Fund(Yonsei Frontier Lab. Young Researcher Supporting Program) of 2017, and by the Hallym University Research Fund , 2017 (HRF-201709-014). Publisher Copyright: © 2017 Elsevier B.V.

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N2 - Immunoassays are used to detect a target analyte by using specific interaction with detection antibodies which form antigen-antibody complexes. In thermophoretic immunoassays the antigen-antibody complexes make slow thermophoresis (movement by thermal stimulation) in comparison with unbound antigens and antibodies. In this work, a sensitive thermophoretic immunoassay was demonstrated by mixing outer membrane (OM) particles of E. coli with autodisplayed Z-domains. In order to compare the influence of OM particles with autodisplayed Z-domains on the sensitivity of thermophoretic immunoassay, the conventional method only with target analyte and detection antibodies was compared with the hyper sensitive thermophoretic immunoassay by mixing OM particles with autodisplayed Z-domains, and the affinity constant was calculated. Finally, the diagnosis of inflammation by detection of a biomarker called C-reactive protein (CRP) was carried out by demonstrating the thermophoretic immunoassay for CRP based on autodisplayedZ-domains could be applied for the medical diagnosis.

AB - Immunoassays are used to detect a target analyte by using specific interaction with detection antibodies which form antigen-antibody complexes. In thermophoretic immunoassays the antigen-antibody complexes make slow thermophoresis (movement by thermal stimulation) in comparison with unbound antigens and antibodies. In this work, a sensitive thermophoretic immunoassay was demonstrated by mixing outer membrane (OM) particles of E. coli with autodisplayed Z-domains. In order to compare the influence of OM particles with autodisplayed Z-domains on the sensitivity of thermophoretic immunoassay, the conventional method only with target analyte and detection antibodies was compared with the hyper sensitive thermophoretic immunoassay by mixing OM particles with autodisplayed Z-domains, and the affinity constant was calculated. Finally, the diagnosis of inflammation by detection of a biomarker called C-reactive protein (CRP) was carried out by demonstrating the thermophoretic immunoassay for CRP based on autodisplayedZ-domains could be applied for the medical diagnosis.

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ER -

Thermophoretic immunoassay based on autodisplayed Z-domains for the diagnosis of C-reactive protein

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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