Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens

Ga Yeon Lee; Ji Hong Bong; Ji Yun Kim; Gu Yoo; Min Park; Min Jung Kang; Joachim Jose; Jae Chul Pyun

doi:10.1016/j.aca.2018.12.038

Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens

Ga Yeon Lee, Ji Hong Bong, Ji Yun Kim, Gu Yoo, Min Park, Min Jung Kang, Joachim Jose, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The autoimmune diseases systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS) are diagnosed by detection of autoantibodies against Ro and La protein autoantigens, respectively. In this work, the diagnosis of autoimmune diseases SLE and SS was demonstrated using thermophoresis of Escherichia coli with the autodisplayed autoantigens. Ro and La protein autoantigens were autodisplayed by constitutive expression together with a fluorescent protein called tdTomato in the cytosol. The binding affinity of the autodisplayed autoantigens was tested against positive and control sera by using FACS as a reference method. The factors influencing interactions between E. coli with autodisplayed autoantigens and autoantibodies in sera during thermophoresis were analyzed by measurement of cell surface charge and size before and after interaction. Finally, the thermophoretic diagnosis of autoimmune diseases SLE and SS was demonstrated using sera from patients afflicted with the respective diseases by estimating sensitivity and selectivity from ROC plots.

Original language	English
Pages (from-to)	106-114
Number of pages	9
Journal	Analytica Chimica Acta
Volume	1055
DOIs	https://doi.org/10.1016/j.aca.2018.12.038
Publication status	Published - 2019 May 9

Bibliographical note

Funding Information:
Funding: This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning and Ministry of Trade, Industry and Energy , Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy , and National Research Foundation of Korea [grant numbers: NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2017R1A6A3A11034081 , NRF-2016R1D1A3B03934169 ]. This work was supported (in part) by the Yonsei University Research Fund (Yonsei Frontier Lab. Young Researcher Supporting Program) of 2018.

Publisher Copyright:
© 2018 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/j.aca.2018.12.038

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title = "Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens",

abstract = "The autoimmune diseases systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS) are diagnosed by detection of autoantibodies against Ro and La protein autoantigens, respectively. In this work, the diagnosis of autoimmune diseases SLE and SS was demonstrated using thermophoresis of Escherichia coli with the autodisplayed autoantigens. Ro and La protein autoantigens were autodisplayed by constitutive expression together with a fluorescent protein called tdTomato in the cytosol. The binding affinity of the autodisplayed autoantigens was tested against positive and control sera by using FACS as a reference method. The factors influencing interactions between E. coli with autodisplayed autoantigens and autoantibodies in sera during thermophoresis were analyzed by measurement of cell surface charge and size before and after interaction. Finally, the thermophoretic diagnosis of autoimmune diseases SLE and SS was demonstrated using sera from patients afflicted with the respective diseases by estimating sensitivity and selectivity from ROC plots.",

author = "Lee, {Ga Yeon} and Bong, {Ji Hong} and Kim, {Ji Yun} and Gu Yoo and Min Park and Kang, {Min Jung} and Joachim Jose and Pyun, {Jae Chul}",

note = "Funding Information: Funding: This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning and Ministry of Trade, Industry and Energy , Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy , and National Research Foundation of Korea [grant numbers: NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2017R1A6A3A11034081 , NRF-2016R1D1A3B03934169 ]. This work was supported (in part) by the Yonsei University Research Fund (Yonsei Frontier Lab. Young Researcher Supporting Program) of 2018. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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AU - Lee, Ga Yeon

AU - Bong, Ji Hong

AU - Kim, Ji Yun

AU - Yoo, Gu

AU - Park, Min

AU - Kang, Min Jung

AU - Jose, Joachim

AU - Pyun, Jae Chul

N1 - Funding Information: Funding: This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning and Ministry of Trade, Industry and Energy , Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy , and National Research Foundation of Korea [grant numbers: NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2017R1A6A3A11034081 , NRF-2016R1D1A3B03934169 ]. This work was supported (in part) by the Yonsei University Research Fund (Yonsei Frontier Lab. Young Researcher Supporting Program) of 2018. Publisher Copyright: © 2018 Elsevier B.V.

PY - 2019/5/9

Y1 - 2019/5/9

N2 - The autoimmune diseases systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS) are diagnosed by detection of autoantibodies against Ro and La protein autoantigens, respectively. In this work, the diagnosis of autoimmune diseases SLE and SS was demonstrated using thermophoresis of Escherichia coli with the autodisplayed autoantigens. Ro and La protein autoantigens were autodisplayed by constitutive expression together with a fluorescent protein called tdTomato in the cytosol. The binding affinity of the autodisplayed autoantigens was tested against positive and control sera by using FACS as a reference method. The factors influencing interactions between E. coli with autodisplayed autoantigens and autoantibodies in sera during thermophoresis were analyzed by measurement of cell surface charge and size before and after interaction. Finally, the thermophoretic diagnosis of autoimmune diseases SLE and SS was demonstrated using sera from patients afflicted with the respective diseases by estimating sensitivity and selectivity from ROC plots.

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Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens

Abstract

Bibliographical note

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