The structural shift of a DNA template between a hairpin and a dimer tunes the emission color of DNA-templated AgNCs

Pratik Shah; Suk Won Choi; Riddhi Nagda; Reka Geczy; Seok Keun Cho; Yong Joo Bhang; Tae Hwan Kim; Tae Yang Song; Phil Hyu Lee; Ju Hee Kang; Peter Waaben Thulstrup; Morten Jannik Bjerrum; Il Lae Jung; Seong Wook Yang

doi:10.1039/c8nr06186f

The structural shift of a DNA template between a hairpin and a dimer tunes the emission color of DNA-templated AgNCs

Pratik Shah, Suk Won Choi, Riddhi Nagda, Reka Geczy, Seok Keun Cho, Yong Joo Bhang, Tae Hwan Kim, Tae Yang Song, Phil Hyu Lee, Ju Hee Kang, Peter Waaben Thulstrup, Morten Jannik Bjerrum, Il Lae Jung, Seong Wook Yang

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19 Citations (Scopus)

Abstract

The scaffolding DNA sequence and the size of silver nanoclusters (AgNCs), confined in a DNA template are the key parameters in determining the fluorescent properties of DNA-stabilized silver nanoclusters (DNA/AgNCs). In addition, we suggest here that the structural shift of a DNA hairpin-dimer is as important as the DNA sequence in determining the emission wavelength of DNA/AgNCs. Furthermore, we show that the structural shift post AgNC formation can be triggered by incubation time and pre-AgNC formation under salt conditions. As an important factor in predicting the emission properties of DNA/AgNCs, the modulation of DNA secondary structures with either sequence changes or ionic conditions can be applied for the dual-color detection system of a target molecule. Particularly, the dual-color detection method may increase the reliability of DNA/AgNC sensors for miRNAs.

Original language	English
Pages (from-to)	20717-20722
Number of pages	6
Journal	Nanoscale
Volume	10
Issue number	44
DOIs	https://doi.org/10.1039/c8nr06186f
Publication status	Published - 2018 Nov 28

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) Projects NRF-2017H1D3A1A01014182 and NRF-2017M2A2A6A04093177 funded by the Ministry of Science, ICT & Future Planning and by Korea Health Technology R&D Project HI16C1118 through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea.

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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Materials Science(all)

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10.1039/c8nr06186f

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Shah, P., Choi, S. W., Nagda, R., Geczy, R., Cho, S. K., Bhang, Y. J., Kim, T. H., Song, T. Y., Lee, P. H., Kang, J. H., Thulstrup, P. W., Bjerrum, M. J., Jung, I. L., & Yang, S. W. (2018). The structural shift of a DNA template between a hairpin and a dimer tunes the emission color of DNA-templated AgNCs. Nanoscale, 10(44), 20717-20722. https://doi.org/10.1039/c8nr06186f

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title = "The structural shift of a DNA template between a hairpin and a dimer tunes the emission color of DNA-templated AgNCs",

abstract = "The scaffolding DNA sequence and the size of silver nanoclusters (AgNCs), confined in a DNA template are the key parameters in determining the fluorescent properties of DNA-stabilized silver nanoclusters (DNA/AgNCs). In addition, we suggest here that the structural shift of a DNA hairpin-dimer is as important as the DNA sequence in determining the emission wavelength of DNA/AgNCs. Furthermore, we show that the structural shift post AgNC formation can be triggered by incubation time and pre-AgNC formation under salt conditions. As an important factor in predicting the emission properties of DNA/AgNCs, the modulation of DNA secondary structures with either sequence changes or ionic conditions can be applied for the dual-color detection system of a target molecule. Particularly, the dual-color detection method may increase the reliability of DNA/AgNC sensors for miRNAs.",

author = "Pratik Shah and Choi, {Suk Won} and Riddhi Nagda and Reka Geczy and Cho, {Seok Keun} and Bhang, {Yong Joo} and Kim, {Tae Hwan} and Song, {Tae Yang} and Lee, {Phil Hyu} and Kang, {Ju Hee} and Thulstrup, {Peter Waaben} and Bjerrum, {Morten Jannik} and Jung, {Il Lae} and Yang, {Seong Wook}",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) Projects NRF-2017H1D3A1A01014182 and NRF-2017M2A2A6A04093177 funded by the Ministry of Science, ICT & Future Planning and by Korea Health Technology R&D Project HI16C1118 through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea. Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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doi = "10.1039/c8nr06186f",

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AU - Shah, Pratik

AU - Choi, Suk Won

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AU - Geczy, Reka

AU - Cho, Seok Keun

AU - Bhang, Yong Joo

AU - Kim, Tae Hwan

AU - Song, Tae Yang

AU - Lee, Phil Hyu

AU - Kang, Ju Hee

AU - Thulstrup, Peter Waaben

AU - Bjerrum, Morten Jannik

AU - Jung, Il Lae

AU - Yang, Seong Wook

N1 - Funding Information: This work was supported by the National Research Foundation (NRF) Projects NRF-2017H1D3A1A01014182 and NRF-2017M2A2A6A04093177 funded by the Ministry of Science, ICT & Future Planning and by Korea Health Technology R&D Project HI16C1118 through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea. Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018/11/28

Y1 - 2018/11/28

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The structural shift of a DNA template between a hairpin and a dimer tunes the emission color of DNA-templated AgNCs

Abstract

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