The serine protease HtrA2/Omi cleaves Parkin and irreversibly inactivates its E3 ubiquitin ligase activity

Hye Min Park; Goo Young Kim; Min Kyung Nam; Geun Hye Seong; Chul Han; Kwang Chul Chung; Seongman Kang; Hyangshuk Rhim

doi:10.1016/j.bbrc.2009.07.079

The serine protease HtrA2/Omi cleaves Parkin and irreversibly inactivates its E3 ubiquitin ligase activity

Hye Min Park, Goo Young Kim, Min Kyung Nam, Geun Hye Seong, Chul Han, Kwang Chul Chung, Seongman Kang, Hyangshuk Rhim

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The serine protease HtrA2 is important in regulating not only apoptosis but also cellular homeostasis. Recently, several lines of evidence suggest that HtrA2 may be intimately associated with Parkin; however, little is known about the functional relationships between HtrA2 and Parkin. Here we have shown that HtrA2 is co-localized with Parkin in the cytosol through the release of HtrA2 from the mitochondria upon cellular stresses. Moreover, endogenous levels of Parkin were significantly decreased in wild-type (HtrA2^+/+) mouse embryonic fibroblasts (MEF) compared with those in HtrA2-knockout (HtrA2^-/-) MEF under the same stress conditions. Using cleavage and binding assays, we have demonstrated that HtrA2 specifically binds to and directly cleaves the E3 ubiquitin (Ub) ligase Parkin. Interestingly, the HtrA2-mediated Parkin cleavage irreversibly disrupts Parkin-mediated synphilin-1 ubiquitination and autoubiquitination, indicating that HtrA2 may play a critical role in the Parkin-related pathway involved in the ubiquitin proteasome system.

Original language	English
Pages (from-to)	537-542
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	387
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2009.07.079
Publication status	Published - 2009 Sept 25

Bibliographical note

Funding Information:
We are grateful to Dr. Julian Downward at Cancer Research UK London Research Institute for offering HtrA2 +/+ and HtrA2 −/− MEF. This work was supported by a grant of the National R&D Program for cancer control, Ministry of Health & Welfare, Republic of Korea (0720300) and the Korea Healthcare technology R&D Project, Ministry of Health, Welfare and Family Affair, Republic of Korea (A080418) and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R01-2008-000-11435-0) and Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2007-314-C00216).

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.07.079

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title = "The serine protease HtrA2/Omi cleaves Parkin and irreversibly inactivates its E3 ubiquitin ligase activity",

abstract = "The serine protease HtrA2 is important in regulating not only apoptosis but also cellular homeostasis. Recently, several lines of evidence suggest that HtrA2 may be intimately associated with Parkin; however, little is known about the functional relationships between HtrA2 and Parkin. Here we have shown that HtrA2 is co-localized with Parkin in the cytosol through the release of HtrA2 from the mitochondria upon cellular stresses. Moreover, endogenous levels of Parkin were significantly decreased in wild-type (HtrA2+/+) mouse embryonic fibroblasts (MEF) compared with those in HtrA2-knockout (HtrA2-/-) MEF under the same stress conditions. Using cleavage and binding assays, we have demonstrated that HtrA2 specifically binds to and directly cleaves the E3 ubiquitin (Ub) ligase Parkin. Interestingly, the HtrA2-mediated Parkin cleavage irreversibly disrupts Parkin-mediated synphilin-1 ubiquitination and autoubiquitination, indicating that HtrA2 may play a critical role in the Parkin-related pathway involved in the ubiquitin proteasome system.",

author = "Park, {Hye Min} and Kim, {Goo Young} and Nam, {Min Kyung} and Seong, {Geun Hye} and Chul Han and Chung, {Kwang Chul} and Seongman Kang and Hyangshuk Rhim",

note = "Funding Information: We are grateful to Dr. Julian Downward at Cancer Research UK London Research Institute for offering HtrA2 +/+ and HtrA2 −/− MEF. This work was supported by a grant of the National R&D Program for cancer control, Ministry of Health & Welfare, Republic of Korea (0720300) and the Korea Healthcare technology R&D Project, Ministry of Health, Welfare and Family Affair, Republic of Korea (A080418) and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R01-2008-000-11435-0) and Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2007-314-C00216). ",

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AU - Kang, Seongman

AU - Rhim, Hyangshuk

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The serine protease HtrA2/Omi cleaves Parkin and irreversibly inactivates its E3 ubiquitin ligase activity

Abstract

Bibliographical note

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