The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a

Se Yeon Lee; Onju Ham; Min Ji Cha; Byeong Wook Song; Eunmi Choi; Il Kwon Kim; Woochul Chang; Soyeon Lim; Chang Youn Lee; Jun Hee Park; Jiyun Lee; Yoonjin Bae; Hyang Hee Seo; Eunhyun Choi; Yangsoo Jang; Ki Chul Hwang

doi:10.1016/j.biomaterials.2012.09.069

The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a

Se Yeon Lee, Onju Ham, Min Ji Cha, Byeong Wook Song, Eunmi Choi, Il Kwon Kim, Woochul Chang, Soyeon Lim, Chang Youn Lee, Jun Hee Park, Jiyun Lee, Yoonjin Bae, Hyang Hee Seo, Eunhyun Choi, Yangsoo Jang, Ki Chul Hwang

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs.

Original language	English
Pages (from-to)	92-99
Number of pages	8
Journal	Biomaterials
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/j.biomaterials.2012.09.069
Publication status	Published - 2013 Jan

Bibliographical note

Funding Information:
This research was supported by a Korea Science and Engineering Foundation Grant funded by the Korea Government (MEST) ( 2011-0019243 , 2011-0019254 ), a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( A120478 ), and a grant from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea ( A085136 ).

All Science Journal Classification (ASJC) codes

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biomaterials.2012.09.069

Cite this

Lee, S. Y., Ham, O., Cha, M. J., Song, B. W., Choi, E., Kim, I. K., Chang, W., Lim, S., Lee, C. Y., Park, J. H., Lee, J., Bae, Y., Seo, H. H., Choi, E., Jang, Y., & Hwang, K. C. (2013). The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a. Biomaterials, 34(1), 92-99. https://doi.org/10.1016/j.biomaterials.2012.09.069

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title = "The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a",

abstract = "Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs.",

author = "Lee, {Se Yeon} and Onju Ham and Cha, {Min Ji} and Song, {Byeong Wook} and Eunmi Choi and Kim, {Il Kwon} and Woochul Chang and Soyeon Lim and Lee, {Chang Youn} and Park, {Jun Hee} and Jiyun Lee and Yoonjin Bae and Seo, {Hyang Hee} and Eunhyun Choi and Yangsoo Jang and Hwang, {Ki Chul}",

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AU - Kim, Il Kwon

AU - Chang, Woochul

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AU - Seo, Hyang Hee

AU - Choi, Eunhyun

AU - Jang, Yangsoo

AU - Hwang, Ki Chul

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AB - Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs.

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The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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