The maintenance of free-living amoebae by cryopreservation.

S. A. Seo, T. S. Yong, K. Im

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7 degree C/min) than by fast cooling (overall 1.3 degrees C/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.

Original languageEnglish
Pages (from-to)151-153
Number of pages3
JournalKisaengch'unghak chapchi. The Korean journal of parasitology
Issue number2
Publication statusPublished - 1992 Jun

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases


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