The Caenorhabditis elegans Cockayne syndrome B protein homologue is encoded by 10 exons of the predicted open reading frame F53H4.1. The gene is expressed in germ cells and all somatic cells of the embryonic to adult stage. Although the gene expression was ubiquitous, its expression level was relatively higher in dividing cells and cells that play fundamental roles in essential physiological functions such as feeding, sensation, and reproduction. RNA interference of the gene hypersensitized C. elegans to UV radiation, as observed in enhanced germ cell proliferation arrest and apoptosis, and increased embryonic lethality, suggesting its role in nucleotide excision repair.
Bibliographical noteFunding Information:
We thank Dr. Yuji Kohara (National institute of Genetics, Japan) for sending us EST clones of csb, Dr. Alan Coulson (Sanger Center) for the F53H4 cosmid clone, and Dr. Andrew Fire (Carnegie Institute) for the pPD95.69 vector. The N2 C. elegans strain was obtained from C. elegans Genetics Center (St. Paul, MN, USA), which is supported by the National Center for Research Resources. This work was supported by Korea Research Foundation Grant KRF-2000-015-DPO325 from the Korean Ministry of Education to I.S.C., H.-S.K., and B.A.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology