TY - JOUR
T1 - The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance
AU - Nam, Eun Ji
AU - Kim, Jae Wook
AU - Kim, Sang Wun
AU - Kim, Young Tae
AU - Kim, Jae Hoon
AU - Yoon, Bo Sung
AU - Cho, Nam Hoon
AU - Kim, Sunghoon
PY - 2007/1
Y1 - 2007/1
N2 - Objectives: The aim of the study was to assess the expression of the Rb pathway (p16INK4a-cdk4-pRb) in cervical intraepithelial neoplasia as a prognostic marker by investigating the association between expression of Rb pathway and the recurrence. Methods: The study group comprised of 265 paraffin-embedded tissues of the uterine cervix collected from patients between 2001 and 2003. Patients underwent ablative or excisional treatment and were followed for 2 years. RB pathway expression was examined using immunohistochemistry applied to a tissue microarray. HPV detection and genotyping were performed with HPV DNA Chip. Statistical analysis was done by ANOVA test, Student's t test, and Pearson's correlation test. Results: With increasing CIN grade, p16INK4a and cdk4 expression rates increased, while the pRb expression rate decreased. The expression rate of p16INK4a was higher (33%) in CIN I with high-risk HPV infection than in CIN I without high-risk HPV infection (19%). The mean expression rates of p16INK4a were 29%, 39%, and 64%, respectively, in CIN I, II, and III without recurrence, but 25%, 28%, and 44% in those with recurrence. The mean expression rates of pRb were 47%, 40%, and 18% in CIN I, II, and III without recurrence, respectively, but 48%, 45%, and 34% in those with recurrence. Conclusion: A relatively low expression rate of p16INK4a has prognostic significance for predicting recurrence. The clinical utility of p16INK4a status for stratifying patients according to their need for aggressive treatment requires further investigation.
AB - Objectives: The aim of the study was to assess the expression of the Rb pathway (p16INK4a-cdk4-pRb) in cervical intraepithelial neoplasia as a prognostic marker by investigating the association between expression of Rb pathway and the recurrence. Methods: The study group comprised of 265 paraffin-embedded tissues of the uterine cervix collected from patients between 2001 and 2003. Patients underwent ablative or excisional treatment and were followed for 2 years. RB pathway expression was examined using immunohistochemistry applied to a tissue microarray. HPV detection and genotyping were performed with HPV DNA Chip. Statistical analysis was done by ANOVA test, Student's t test, and Pearson's correlation test. Results: With increasing CIN grade, p16INK4a and cdk4 expression rates increased, while the pRb expression rate decreased. The expression rate of p16INK4a was higher (33%) in CIN I with high-risk HPV infection than in CIN I without high-risk HPV infection (19%). The mean expression rates of p16INK4a were 29%, 39%, and 64%, respectively, in CIN I, II, and III without recurrence, but 25%, 28%, and 44% in those with recurrence. The mean expression rates of pRb were 47%, 40%, and 18% in CIN I, II, and III without recurrence, respectively, but 48%, 45%, and 34% in those with recurrence. Conclusion: A relatively low expression rate of p16INK4a has prognostic significance for predicting recurrence. The clinical utility of p16INK4a status for stratifying patients according to their need for aggressive treatment requires further investigation.
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U2 - 10.1016/j.ygyno.2006.07.043
DO - 10.1016/j.ygyno.2006.07.043
M3 - Article
C2 - 17046054
AN - SCOPUS:33845969200
SN - 0090-8258
VL - 104
SP - 207
EP - 211
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 1
ER -