TY - JOUR
T1 - The expression of TLR3 and cytokines induced by poly I:C in human retinal pigment epithelial cells
AU - Sun, Ju Choi
AU - Kim, Jongwook
AU - Su, Jung Park
AU - Hyun, Sook Park
AU - Lee, Kyoung Ho
AU - Kim, Soo Ki
AU - Joo, Young Park
PY - 2007
Y1 - 2007
N2 - In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-α, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-β mRNA expression and 2′,5′-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.
AB - In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-α, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-β mRNA expression and 2′,5′-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.
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U2 - 10.4167/jbv.2007.37.4.241
DO - 10.4167/jbv.2007.37.4.241
M3 - Article
AN - SCOPUS:44849094826
SN - 1598-2467
VL - 37
SP - 241
EP - 248
JO - Journal of Bacteriology and Virology
JF - Journal of Bacteriology and Virology
IS - 4
ER -