Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.
|Number of pages||7|
|Journal||Molecular Biology Reports|
|Publication status||Published - 2012 Feb|
Bibliographical noteFunding Information:
Acknowledgments We would particularly like to thank Kevin M. Ryan for providing the DRAM expression plasmid and T. Yoshimori for providing the GFP-LC3 plasmid. This study was supported by a National Research Foundation of Korea Grant funded by the Korean
All Science Journal Classification (ASJC) codes
- Molecular Biology