The apolipoprotein(a) size, pentanucleotide repeat, C/T(+93) polymorphisms of apolipoprotein(a) gene, serum lipoprotein(a) concentrations and their relationship in a Korean population

Jeong Ho Kim; Kyung Ho Roh; Sang Min Nam; Hyun Young Park; Yangsoo Jang; Dong Kee Kim; Kyung Soon Song

doi:10.1016/S0009-8981(01)00683-0

The apolipoprotein(a) size, pentanucleotide repeat, C/T(+93) polymorphisms of apolipoprotein(a) gene, serum lipoprotein(a) concentrations and their relationship in a Korean population

Jeong Ho Kim, Kyung Ho Roh, Sang Min Nam, Hyun Young Park, Yangsoo Jang, Dong Kee Kim, Kyung Soon Song

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background: In addition to apolipoprotein(a) [apo(a)] kringle 4 variable number of tandem repeat (K4-VNTR), pentanucleotide repeat polymorphism (PNRP) and C/T(+ 93) polymorphism [C/T(+ 93)] of apo(a) gene have been suggested to be related to lipoprotein(a) [Lp(a)] concentration. We studied the distribution of these genetic polymorphisms and their relationship with Lp(a) concentrations in a Korean population. Methods: One hundred thirty-two Korean adults were examined. Lp(a) was measured with enzyme-linked immunosorbent assay (ELISA). Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. PNRP was measured after DNA amplification. The C/T(+ 93) ratio was measured by a amplification refractory mutation system. Results: Lp(a) was inversely correlated with K4-VNTR (r = 0.732, p < 0.0001), but was associated neither with any PNRP haplotype nor with C/T(+ 93) by multiple regression analysis, although we found a significant decrease of Lp(a) in PNRP 9/9 individuals (p < 0.01). There was a strong linkage disequilibrium between 9 haplotypes of PNRP and the T haplotype of C/T(+ 93). Conclusions: Inverse relationship between serum Lp(a) and K4 number of apo(a) was confirmed in normal Korean adults. PNRP 9/9 genotype appeared to have a reducing effect on Lp(a), but neither 9 haplotype heterozygotes of PNRP nor the T haplotype C/T(+ 93) affected Lp(a) concentrations in Koreans.

Original language	English
Pages (from-to)	113-123
Number of pages	11
Journal	Clinica Chimica Acta
Volume	314
Issue number	1-2
DOIs	https://doi.org/10.1016/S0009-8981(01)00683-0
Publication status	Published - 2001

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (HMP-97-M-1-0011). We are grateful for resident doctors and phlebotomists of department of clinical pathology in Severance hospital for their assistance in collecting samples. We are indebted to Dr. Won Ki Min, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea for his kind donation of apo(a) isoform standard mixture (Immuno, Vienna, Austria). We are also grateful to Young Sook Park, PhD for her excellent technical support, Sun Ha Jee, PhD and Ki Jun Song, MS, for their statistical assistance.

All Science Journal Classification (ASJC) codes

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/S0009-8981(01)00683-0

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@article{9862c5105ecc4fceac0d70ba617009c4,

title = "The apolipoprotein(a) size, pentanucleotide repeat, C/T(+93) polymorphisms of apolipoprotein(a) gene, serum lipoprotein(a) concentrations and their relationship in a Korean population",

abstract = "Background: In addition to apolipoprotein(a) [apo(a)] kringle 4 variable number of tandem repeat (K4-VNTR), pentanucleotide repeat polymorphism (PNRP) and C/T(+ 93) polymorphism [C/T(+ 93)] of apo(a) gene have been suggested to be related to lipoprotein(a) [Lp(a)] concentration. We studied the distribution of these genetic polymorphisms and their relationship with Lp(a) concentrations in a Korean population. Methods: One hundred thirty-two Korean adults were examined. Lp(a) was measured with enzyme-linked immunosorbent assay (ELISA). Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. PNRP was measured after DNA amplification. The C/T(+ 93) ratio was measured by a amplification refractory mutation system. Results: Lp(a) was inversely correlated with K4-VNTR (r = 0.732, p < 0.0001), but was associated neither with any PNRP haplotype nor with C/T(+ 93) by multiple regression analysis, although we found a significant decrease of Lp(a) in PNRP 9/9 individuals (p < 0.01). There was a strong linkage disequilibrium between 9 haplotypes of PNRP and the T haplotype of C/T(+ 93). Conclusions: Inverse relationship between serum Lp(a) and K4 number of apo(a) was confirmed in normal Korean adults. PNRP 9/9 genotype appeared to have a reducing effect on Lp(a), but neither 9 haplotype heterozygotes of PNRP nor the T haplotype C/T(+ 93) affected Lp(a) concentrations in Koreans.",

author = "Kim, {Jeong Ho} and Roh, {Kyung Ho} and Nam, {Sang Min} and Park, {Hyun Young} and Yangsoo Jang and Kim, {Dong Kee} and Song, {Kyung Soon}",

note = "Funding Information: This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (HMP-97-M-1-0011). We are grateful for resident doctors and phlebotomists of department of clinical pathology in Severance hospital for their assistance in collecting samples. We are indebted to Dr. Won Ki Min, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea for his kind donation of apo(a) isoform standard mixture (Immuno, Vienna, Austria). We are also grateful to Young Sook Park, PhD for her excellent technical support, Sun Ha Jee, PhD and Ki Jun Song, MS, for their statistical assistance.",

year = "2001",

doi = "10.1016/S0009-8981(01)00683-0",

language = "English",

volume = "314",

pages = "113--123",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - The apolipoprotein(a) size, pentanucleotide repeat, C/T(+93) polymorphisms of apolipoprotein(a) gene, serum lipoprotein(a) concentrations and their relationship in a Korean population

AU - Kim, Jeong Ho

AU - Roh, Kyung Ho

AU - Nam, Sang Min

AU - Park, Hyun Young

AU - Jang, Yangsoo

AU - Kim, Dong Kee

AU - Song, Kyung Soon

N1 - Funding Information: This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (HMP-97-M-1-0011). We are grateful for resident doctors and phlebotomists of department of clinical pathology in Severance hospital for their assistance in collecting samples. We are indebted to Dr. Won Ki Min, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea for his kind donation of apo(a) isoform standard mixture (Immuno, Vienna, Austria). We are also grateful to Young Sook Park, PhD for her excellent technical support, Sun Ha Jee, PhD and Ki Jun Song, MS, for their statistical assistance.

PY - 2001

Y1 - 2001

N2 - Background: In addition to apolipoprotein(a) [apo(a)] kringle 4 variable number of tandem repeat (K4-VNTR), pentanucleotide repeat polymorphism (PNRP) and C/T(+ 93) polymorphism [C/T(+ 93)] of apo(a) gene have been suggested to be related to lipoprotein(a) [Lp(a)] concentration. We studied the distribution of these genetic polymorphisms and their relationship with Lp(a) concentrations in a Korean population. Methods: One hundred thirty-two Korean adults were examined. Lp(a) was measured with enzyme-linked immunosorbent assay (ELISA). Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. PNRP was measured after DNA amplification. The C/T(+ 93) ratio was measured by a amplification refractory mutation system. Results: Lp(a) was inversely correlated with K4-VNTR (r = 0.732, p < 0.0001), but was associated neither with any PNRP haplotype nor with C/T(+ 93) by multiple regression analysis, although we found a significant decrease of Lp(a) in PNRP 9/9 individuals (p < 0.01). There was a strong linkage disequilibrium between 9 haplotypes of PNRP and the T haplotype of C/T(+ 93). Conclusions: Inverse relationship between serum Lp(a) and K4 number of apo(a) was confirmed in normal Korean adults. PNRP 9/9 genotype appeared to have a reducing effect on Lp(a), but neither 9 haplotype heterozygotes of PNRP nor the T haplotype C/T(+ 93) affected Lp(a) concentrations in Koreans.

AB - Background: In addition to apolipoprotein(a) [apo(a)] kringle 4 variable number of tandem repeat (K4-VNTR), pentanucleotide repeat polymorphism (PNRP) and C/T(+ 93) polymorphism [C/T(+ 93)] of apo(a) gene have been suggested to be related to lipoprotein(a) [Lp(a)] concentration. We studied the distribution of these genetic polymorphisms and their relationship with Lp(a) concentrations in a Korean population. Methods: One hundred thirty-two Korean adults were examined. Lp(a) was measured with enzyme-linked immunosorbent assay (ELISA). Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. PNRP was measured after DNA amplification. The C/T(+ 93) ratio was measured by a amplification refractory mutation system. Results: Lp(a) was inversely correlated with K4-VNTR (r = 0.732, p < 0.0001), but was associated neither with any PNRP haplotype nor with C/T(+ 93) by multiple regression analysis, although we found a significant decrease of Lp(a) in PNRP 9/9 individuals (p < 0.01). There was a strong linkage disequilibrium between 9 haplotypes of PNRP and the T haplotype of C/T(+ 93). Conclusions: Inverse relationship between serum Lp(a) and K4 number of apo(a) was confirmed in normal Korean adults. PNRP 9/9 genotype appeared to have a reducing effect on Lp(a), but neither 9 haplotype heterozygotes of PNRP nor the T haplotype C/T(+ 93) affected Lp(a) concentrations in Koreans.

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U2 - 10.1016/S0009-8981(01)00683-0

DO - 10.1016/S0009-8981(01)00683-0

M3 - Article

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AN - SCOPUS:0035170564

SN - 0009-8981

VL - 314

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JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

The apolipoprotein(a) size, pentanucleotide repeat, C/T(+93) polymorphisms of apolipoprotein(a) gene, serum lipoprotein(a) concentrations and their relationship in a Korean population

Abstract

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