We have measured the temperature dependence of the capacitance on the poly(dA), poly(dA)-poly(dT), and DNA solutions with both A-T and G-C base pairs to investigate the relation between the capacitance and the DNA structure. As the temperature increases, the capacitance of the single-stranded DNA solution increases, whereas that of the double-stranded one decreases with increasing the temperature and exhibits an upturn when DNA molecules are denatured. Moreover, the temperature dependence of the capacitance is found to be different depending on the DNA sequence. These results suggest that the conformational change of DNA molecules can be detected through the capacitance measurements that do not require any labeling and immobilization on a solid support.
Bibliographical noteFunding Information:
This work was financially supported by Yonsei University and KOSEF through National Core Research Center for Nanomedical Technology.
All Science Journal Classification (ASJC) codes
- General Materials Science
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering