Surface Plasmon Localization-Based Super-resolved Raman Microscopy

Hongki Lee; Kyungnam Kang; Kentaro Mochizuki; Changhun Lee; Kar Ann Toh; Seung Ah Lee; Katsumasa Fujita; Donghyun Kim

doi:10.1021/acs.nanolett.0c04219

Surface Plasmon Localization-Based Super-resolved Raman Microscopy

Hongki Lee, Kyungnam Kang, Kentaro Mochizuki, Changhun Lee, Kar Ann Toh, Seung Ah Lee, Katsumasa Fujita, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

We investigate label-free measurement of molecular distribution by super-resolved Raman microscopy using surface plasmon (SP) localization. Localized SP was formed with plasmonic nanopost arrays (PNAs) for measurement of the molecular distribution in HeLa cells. Compared with conventional Raman microscopy on gold thin films, PNAs induce a localized near-field, which allows for the enhancement of the peak signal-to-noise ratio by as much as 4.5 dB in the Raman shifts. Super-resolved distributions of aromatic amino acids and lipids (C-C stretching and C-H2 twist mode) as targets in HeLa cells were obtained after image reconstruction. Results show almost 4-fold improvement on average in the lateral precision over conventional diffraction-limited Raman microscopy images. Combined with axial imaging in an evanescent field, the results suggest an improvement in optical resolution due to superlocalized light volume by more than an order of magnitude over that of conventional diffraction-limited Raman microscopy.

Original language	English
Pages (from-to)	8951-8958
Number of pages	8
Journal	Nano letters
Volume	20
Issue number	12
DOIs	https://doi.org/10.1021/acs.nanolett.0c04219
Publication status	Published - 2020 Dec 9

Bibliographical note

Funding Information:
This work was supported by National Research Foundation grants funded by the Korean Government (2019R1A4A1025958, 2019K2A9A2A08000198, and 2019R1A6A3A01096804) and partly sponsored by the Ministry of Trade, Industry and Energy (P048000064). We acknowledge Woo Joong Rhee and Prof. Jeon-Soo Shin at the Department of Microbiology, School of Medicine, Yonsei University for preparing HeLa cells for SEM on PNAs.

Publisher Copyright:
© 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.0c04219

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title = "Surface Plasmon Localization-Based Super-resolved Raman Microscopy",

abstract = "We investigate label-free measurement of molecular distribution by super-resolved Raman microscopy using surface plasmon (SP) localization. Localized SP was formed with plasmonic nanopost arrays (PNAs) for measurement of the molecular distribution in HeLa cells. Compared with conventional Raman microscopy on gold thin films, PNAs induce a localized near-field, which allows for the enhancement of the peak signal-to-noise ratio by as much as 4.5 dB in the Raman shifts. Super-resolved distributions of aromatic amino acids and lipids (C-C stretching and C-H2 twist mode) as targets in HeLa cells were obtained after image reconstruction. Results show almost 4-fold improvement on average in the lateral precision over conventional diffraction-limited Raman microscopy images. Combined with axial imaging in an evanescent field, the results suggest an improvement in optical resolution due to superlocalized light volume by more than an order of magnitude over that of conventional diffraction-limited Raman microscopy.",

author = "Hongki Lee and Kyungnam Kang and Kentaro Mochizuki and Changhun Lee and Toh, {Kar Ann} and Lee, {Seung Ah} and Katsumasa Fujita and Donghyun Kim",

note = "Funding Information: This work was supported by National Research Foundation grants funded by the Korean Government (2019R1A4A1025958, 2019K2A9A2A08000198, and 2019R1A6A3A01096804) and partly sponsored by the Ministry of Trade, Industry and Energy (P048000064). We acknowledge Woo Joong Rhee and Prof. Jeon-Soo Shin at the Department of Microbiology, School of Medicine, Yonsei University for preparing HeLa cells for SEM on PNAs. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

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AU - Lee, Seung Ah

AU - Fujita, Katsumasa

AU - Kim, Donghyun

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PY - 2020/12/9

Y1 - 2020/12/9

N2 - We investigate label-free measurement of molecular distribution by super-resolved Raman microscopy using surface plasmon (SP) localization. Localized SP was formed with plasmonic nanopost arrays (PNAs) for measurement of the molecular distribution in HeLa cells. Compared with conventional Raman microscopy on gold thin films, PNAs induce a localized near-field, which allows for the enhancement of the peak signal-to-noise ratio by as much as 4.5 dB in the Raman shifts. Super-resolved distributions of aromatic amino acids and lipids (C-C stretching and C-H2 twist mode) as targets in HeLa cells were obtained after image reconstruction. Results show almost 4-fold improvement on average in the lateral precision over conventional diffraction-limited Raman microscopy images. Combined with axial imaging in an evanescent field, the results suggest an improvement in optical resolution due to superlocalized light volume by more than an order of magnitude over that of conventional diffraction-limited Raman microscopy.

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Surface Plasmon Localization-Based Super-resolved Raman Microscopy

Abstract

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