Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica

Becky M. Flores; Samuel L. Stanley; Tai Soon Yong; Manzoor Ali; Wengang Yang; Dana L. Diedrich; Bruce E. Torian

doi:10.1093/infdis/173.1.226

Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica

Becky M. Flores, Samuel L. Stanley, Tai Soon Yong, Manzoor Ali, Wengang Yang, Dana L. Diedrich, Bruce E. Torian

Department of Environmental Medical Biology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.

Original language	English
Pages (from-to)	226-231
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	173
Issue number	1
DOIs	https://doi.org/10.1093/infdis/173.1.226
Publication status	Published - 1996

Bibliographical note

Funding Information:
Received 12 July 1995; revised 30 August 1995. Financial support: National Institutes of Health (AI-28188 to B.E.T.; AI-37977 and AI-01231 [Public Health Service Career Development Award] to S.L.S.); Monsanto Company (to S.L.S.). Reprints or correspondence: Dr. Bruce E. Torian, Idaho State University, College of Pharmacy, Dept. of Pharmaceutical Sciences, 970 S. 5th Ave., Pocatello, ID 83209-8334.

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/173.1.226

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title = "Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica",

abstract = "The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.",

author = "Flores, {Becky M.} and Stanley, {Samuel L.} and Yong, {Tai Soon} and Manzoor Ali and Wengang Yang and Diedrich, {Dana L.} and Torian, {Bruce E.}",

note = "Funding Information: Received 12 July 1995; revised 30 August 1995. Financial support: National Institutes of Health (AI-28188 to B.E.T.; AI-37977 and AI-01231 [Public Health Service Career Development Award] to S.L.S.); Monsanto Company (to S.L.S.). Reprints or correspondence: Dr. Bruce E. Torian, Idaho State University, College of Pharmacy, Dept. of Pharmaceutical Sciences, 970 S. 5th Ave., Pocatello, ID 83209-8334.",

year = "1996",

doi = "10.1093/infdis/173.1.226",

language = "English",

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AU - Flores, Becky M.

AU - Stanley, Samuel L.

AU - Yong, Tai Soon

AU - Ali, Manzoor

AU - Yang, Wengang

AU - Diedrich, Dana L.

AU - Torian, Bruce E.

N1 - Funding Information: Received 12 July 1995; revised 30 August 1995. Financial support: National Institutes of Health (AI-28188 to B.E.T.; AI-37977 and AI-01231 [Public Health Service Career Development Award] to S.L.S.); Monsanto Company (to S.L.S.). Reprints or correspondence: Dr. Bruce E. Torian, Idaho State University, College of Pharmacy, Dept. of Pharmaceutical Sciences, 970 S. 5th Ave., Pocatello, ID 83209-8334.

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Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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