The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.
Bibliographical noteFunding Information:
Received 12 July 1995; revised 30 August 1995. Financial support: National Institutes of Health (AI-28188 to B.E.T.; AI-37977 and AI-01231 [Public Health Service Career Development Award] to S.L.S.); Monsanto Company (to S.L.S.). Reprints or correspondence: Dr. Bruce E. Torian, Idaho State University, College of Pharmacy, Dept. of Pharmaceutical Sciences, 970 S. 5th Ave., Pocatello, ID 83209-8334.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases