Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization

Seyoung Moon, Dong Jun Kim, Kyujung Kim, Donghyun Kim, Hosub Lee, Kangtaek Lee, Seungjoo Haam

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


We have investigated surface-enhanced plasmon resonance detection of DNA hybridization. Surface enhancement was based on the excitation of localized surface plasmon using subwavelength nanograt- ings, at a 300 nm period, coated with 24-mer ssDNA oligonucleotide, while optical signatures of DNA were amplified at the same time by gold nanoparticles conjugated with complementary ssDNA strands. When using nanoparticles of different sizes, maximum sensitivity enhancement, of more than 18 times, was obtained with nanoparticles of 20 nm diameter. This enhancement is mainly due to nanoparticle- associated signal amplification. Additional surface enhancement boosted the detection sensitivity by 57%. We have also confirmed the sensitivity enhancement to be linearly related to nanoparticle volume.

Original languageEnglish
Pages (from-to)484-491
Number of pages8
JournalApplied Optics
Issue number3
Publication statusPublished - 2010 Jan 20

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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