Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Mi Young Byun; Li Hua Cui; Woo Taek Kim

doi:10.1016/j.bbrc.2015.11.044

Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Mi Young Byun, Li Hua Cui, Woo Taek Kim

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3 Citations (Scopus)

Abstract

The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

Original language	English
Pages (from-to)	857-862
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	468
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2015.11.044
Publication status	Published - 2015 Dec 25

Bibliographical note

Funding Information:
This work was supported by a grant from the Woo Jang Chun Special Project (PJ009106) funded by the Rural Development Administration, Republic of Korea , to W.T.K.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserve.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2015.11.044

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title = "Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)",

abstract = "The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.",

author = "Byun, {Mi Young} and Cui, {Li Hua} and Kim, {Woo Taek}",

note = "Funding Information: This work was supported by a grant from the Woo Jang Chun Special Project (PJ009106) funded by the Rural Development Administration, Republic of Korea , to W.T.K. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserve.",

year = "2015",

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doi = "10.1016/j.bbrc.2015.11.044",

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AU - Byun, Mi Young

AU - Cui, Li Hua

AU - Kim, Woo Taek

N1 - Funding Information: This work was supported by a grant from the Woo Jang Chun Special Project (PJ009106) funded by the Rural Development Administration, Republic of Korea , to W.T.K. Publisher Copyright: © 2015 Elsevier Inc. All rights reserve.

PY - 2015/12/25

Y1 - 2015/12/25

N2 - The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

AB - The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

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Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Abstract

Bibliographical note

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