Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility

Hongki Lee; Hajun Yoo; Gwiyeong Moon; Kar Ann Toh; Kentaro Mochizuki; Katsumasa Fujita; Donghyun Kim

doi:10.1063/5.0064082

Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility

Hongki Lee, Hajun Yoo, Gwiyeong Moon, Kar Ann Toh, Kentaro Mochizuki, Katsumasa Fujita, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm-1 were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.

Original language	English
Article number	144202
Journal	Journal of Chemical Physics
Volume	155
Issue number	14
DOIs	https://doi.org/10.1063/5.0064082
Publication status	Published - 2021 Oct 14

Bibliographical note

Publisher Copyright:
© 2021 Author(s).

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)
Physical and Theoretical Chemistry

Access to Document

10.1063/5.0064082

Cite this

@article{1c61f47543d0478bbb46e6737fe7db9b,

title = "Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility",

abstract = "In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm-1 were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.",

author = "Hongki Lee and Hajun Yoo and Gwiyeong Moon and Toh, {Kar Ann} and Kentaro Mochizuki and Katsumasa Fujita and Donghyun Kim",

note = "Publisher Copyright: {\textcopyright} 2021 Author(s).",

year = "2021",

month = oct,

day = "14",

doi = "10.1063/5.0064082",

language = "English",

volume = "155",

journal = "Journal of Chemical Physics",

issn = "0021-9606",

publisher = "American Institute of Physics Publising LLC",

number = "14",

}

TY - JOUR

T1 - Super-resolved Raman microscopy using random structured light illumination

T2 - Concept and feasibility

AU - Lee, Hongki

AU - Yoo, Hajun

AU - Moon, Gwiyeong

AU - Toh, Kar Ann

AU - Mochizuki, Kentaro

AU - Fujita, Katsumasa

AU - Kim, Donghyun

PY - 2021/10/14

Y1 - 2021/10/14

N2 - In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm-1 were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.

AB - In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm-1 were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.

UR - http://www.scopus.com/inward/record.url?scp=85117160337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85117160337&partnerID=8YFLogxK

U2 - 10.1063/5.0064082

DO - 10.1063/5.0064082

M3 - Article

C2 - 34654313

AN - SCOPUS:85117160337

SN - 0021-9606

VL - 155

JO - Journal of Chemical Physics

JF - Journal of Chemical Physics

IS - 14

M1 - 144202

ER -

Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this