Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences

Andrea E. Holmes; Jung Kyu Choi; Jacob Francis; Alessandro D'Urso; Milan Balaz

doi:10.1016/j.jinorgbio.2012.02.001

Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences

Andrea E. Holmes, Jung Kyu Choi, Jacob Francis, Alessandro D'Urso, Milan Balaz

Integrated Sciences and Engineering Division

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine ^{4 +} allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).

Original language	English
Pages (from-to)	18-20
Number of pages	3
Journal	Journal of Inorganic Biochemistry
Volume	110
DOIs	https://doi.org/10.1016/j.jinorgbio.2012.02.001
Publication status	Published - 2012 May

Bibliographical note

Funding Information:
MB thanks the University of Wyoming Start-Up Fund. AEH thanks the National Center for Research Resources ( 5P20RR016469 ) and the National Institute for General Medical Science ( 8P20GM103427 ), a component of the National Institutes of Health (NIH) , and the NSF-EPSCoR-EPS-1004094 and the NSF CHE-0747949 .

All Science Journal Classification (ASJC) codes

Biochemistry
Inorganic Chemistry

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10.1016/j.jinorgbio.2012.02.001

Cite this

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title = "Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences",

abstract = "We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine 4 + allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).",

author = "Holmes, {Andrea E.} and Choi, {Jung Kyu} and Jacob Francis and Alessandro D'Urso and Milan Balaz",

note = "Funding Information: MB thanks the University of Wyoming Start-Up Fund. AEH thanks the National Center for Research Resources ( 5P20RR016469 ) and the National Institute for General Medical Science ( 8P20GM103427 ), a component of the National Institutes of Health (NIH) , and the NSF-EPSCoR-EPS-1004094 and the NSF CHE-0747949 . ",

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doi = "10.1016/j.jinorgbio.2012.02.001",

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T1 - Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences

AU - Holmes, Andrea E.

AU - Choi, Jung Kyu

AU - Francis, Jacob

AU - D'Urso, Alessandro

AU - Balaz, Milan

N1 - Funding Information: MB thanks the University of Wyoming Start-Up Fund. AEH thanks the National Center for Research Resources ( 5P20RR016469 ) and the National Institute for General Medical Science ( 8P20GM103427 ), a component of the National Institutes of Health (NIH) , and the NSF-EPSCoR-EPS-1004094 and the NSF CHE-0747949 .

PY - 2012/5

Y1 - 2012/5

N2 - We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine 4 + allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).

AB - We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine 4 + allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).

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ER -

Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences

Abstract

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