Structure and function of the potent cyclic and linear melanocortin analogues

Min Kyu Cho; Chul Jin Lee; Chang Hun Lee; Song Zhe Li; Sung Kil Lim; Ja Hyun Baik; Weontae Lee

doi:10.1016/j.jsb.2005.03.008

Structure and function of the potent cyclic and linear melanocortin analogues

Min Kyu Cho, Chul Jin Lee, Chang Hun Lee, Song Zhe Li, Sung Kil Lim, Ja Hyun Baik, Weontae Lee

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The MC3R and MC4R proteins comprise two melanocortin receptor subtypes that are involved in obesity, with each protein displaying a unique mechanism of action. To enable the design of a selective drug candidate, the solution structures of four peptidyl analogues of the melanocyte stimulating hormones, NDP-MSH, NDP-MSH(4-10) and two cyclic forms ([C5,C10]NDP-MSH(5-10), [C5,C10]NDP-MSH(5-11)), were characterized by two-dimensional nuclear magnetic resonance (NMR) spectroscopy and simulated annealing calculations. Using data from c-AMP assays in combination with structural analysis of melanocortin receptor/ligand models, we conclude that a lysine residue at the C-terminus of the His-Phe-Arg-Trp core sequence of melanocortin hormone is an important determinant for receptor selectivity in the both cyclic and linear MSH analogues. Our results suggest that side-chain orientation and charge-charge interactions with the ligand molecule play critical roles in receptor selectivity, whereas the overall backbone conformation or turn type contributes mainly to receptor binding.

Original language	English
Pages (from-to)	300-308
Number of pages	9
Journal	Journal of Structural Biology
Volume	150
Issue number	3
DOIs	https://doi.org/10.1016/j.jsb.2005.03.008
Publication status	Published - 2005 Jun

Bibliographical note

Funding Information:
This work was supported by research grants from the Korean Ministry of Health and Welfare (01-PJ1-PG1-CH05-0005). This work was also supported by the NRL program of MOST NRDP (M1-0203-00-0020) and Korea Science and Engineering Foundation (KOSEF) through Protein Network Research Center at Yonsei University and in part by the Brain Korea 21 Project (W.L.).

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Structural Biology

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10.1016/j.jsb.2005.03.008

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title = "Structure and function of the potent cyclic and linear melanocortin analogues",

abstract = "The MC3R and MC4R proteins comprise two melanocortin receptor subtypes that are involved in obesity, with each protein displaying a unique mechanism of action. To enable the design of a selective drug candidate, the solution structures of four peptidyl analogues of the melanocyte stimulating hormones, NDP-MSH, NDP-MSH(4-10) and two cyclic forms ([C5,C10]NDP-MSH(5-10), [C5,C10]NDP-MSH(5-11)), were characterized by two-dimensional nuclear magnetic resonance (NMR) spectroscopy and simulated annealing calculations. Using data from c-AMP assays in combination with structural analysis of melanocortin receptor/ligand models, we conclude that a lysine residue at the C-terminus of the His-Phe-Arg-Trp core sequence of melanocortin hormone is an important determinant for receptor selectivity in the both cyclic and linear MSH analogues. Our results suggest that side-chain orientation and charge-charge interactions with the ligand molecule play critical roles in receptor selectivity, whereas the overall backbone conformation or turn type contributes mainly to receptor binding.",

author = "Cho, {Min Kyu} and Lee, {Chul Jin} and Lee, {Chang Hun} and Li, {Song Zhe} and Lim, {Sung Kil} and Baik, {Ja Hyun} and Weontae Lee",

note = "Funding Information: This work was supported by research grants from the Korean Ministry of Health and Welfare (01-PJ1-PG1-CH05-0005). This work was also supported by the NRL program of MOST NRDP (M1-0203-00-0020) and Korea Science and Engineering Foundation (KOSEF) through Protein Network Research Center at Yonsei University and in part by the Brain Korea 21 Project (W.L.).",

year = "2005",

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AU - Cho, Min Kyu

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AU - Lee, Chang Hun

AU - Li, Song Zhe

AU - Lim, Sung Kil

AU - Baik, Ja Hyun

AU - Lee, Weontae

N1 - Funding Information: This work was supported by research grants from the Korean Ministry of Health and Welfare (01-PJ1-PG1-CH05-0005). This work was also supported by the NRL program of MOST NRDP (M1-0203-00-0020) and Korea Science and Engineering Foundation (KOSEF) through Protein Network Research Center at Yonsei University and in part by the Brain Korea 21 Project (W.L.).

PY - 2005/6

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N2 - The MC3R and MC4R proteins comprise two melanocortin receptor subtypes that are involved in obesity, with each protein displaying a unique mechanism of action. To enable the design of a selective drug candidate, the solution structures of four peptidyl analogues of the melanocyte stimulating hormones, NDP-MSH, NDP-MSH(4-10) and two cyclic forms ([C5,C10]NDP-MSH(5-10), [C5,C10]NDP-MSH(5-11)), were characterized by two-dimensional nuclear magnetic resonance (NMR) spectroscopy and simulated annealing calculations. Using data from c-AMP assays in combination with structural analysis of melanocortin receptor/ligand models, we conclude that a lysine residue at the C-terminus of the His-Phe-Arg-Trp core sequence of melanocortin hormone is an important determinant for receptor selectivity in the both cyclic and linear MSH analogues. Our results suggest that side-chain orientation and charge-charge interactions with the ligand molecule play critical roles in receptor selectivity, whereas the overall backbone conformation or turn type contributes mainly to receptor binding.

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Structure and function of the potent cyclic and linear melanocortin analogues

Abstract

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