Structural study of monomethyl fumarate-bound human gapdh

Jun Bae Park, Hayeong Park, Jimin Son, Sang Jun Ha, Hyun Soo Cho

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a core enzyme of the aerobic glycolytic pathway with versatile functions and is associated with cancer development. Recently, Kornberg et al. published the detailed correlation between GAPDH and di-or monomethyl fumarate (DMF or MMF), which are well-known GAPDH antagonists in the immune system. As an extension, herein, we report the crystal structure of MMF-bound human GAPDH at 2.29 Å. The MMF molecule is covalently linked to the catalytic Cys152 of human GAPDH, and inhibits the catalytic activity of the residue and dramatically reduces the enzymatic activity of GAPDH. Structural comparisons between NAD+-bound GAPDH and MMF-bound GAPDH revealed that the covalently linked MMF can block the binding of the NAD+ co-substrate due to steric hindrance of the nicotinamide portion of the NAD+ molecule, illuminating the specific mechanism by which MMF inhibits GAPDH. Our data provide insights into GAPDH antagonist development for GAPDH-mediated disease treatment.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalMolecules and cells
Issue number8
Publication statusPublished - 2019

Bibliographical note

Publisher Copyright:
© The Korean Society for Molecular and Cellular Biology. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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