Structural influence on the post-clustering stability of DNA/AgNCs fluorescence

Riddhi Nagda, Pratik Shah, Chang Seop Lee, Sooyeon Park, Seong Wook Yang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates.

Original languageEnglish
Article number667
Issue number5
Publication statusPublished - 2019 May

Bibliographical note

Publisher Copyright:
© 2019 by the authors.

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Materials Science(all)


Dive into the research topics of 'Structural influence on the post-clustering stability of DNA/AgNCs fluorescence'. Together they form a unique fingerprint.

Cite this