Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Song Yee Kim; Young Ae Kang; Il Kwon Bae; Jae Joon Yim; Moo Suk Park; Young Sam Kim; Se Kyu Kim; Joon Chang; Seok Hoon Jeong

doi:10.1016/j.diagmicrobio.2013.06.023

Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Song Yee Kim, Young Ae Kang, Il Kwon Bae, Jae Joon Yim, Moo Suk Park, Young Sam Kim, Se Kyu Kim, Joon Chang, Seok Hoon Jeong

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.06.023
Publication status	Published - 2013 Oct

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (2011-0013018).

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2013.06.023

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title = "Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense",

abstract = "This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.",

author = "Kim, {Song Yee} and Kang, {Young Ae} and Bae, {Il Kwon} and Yim, {Jae Joon} and Park, {Moo Suk} and Kim, {Young Sam} and Kim, {Se Kyu} and Joon Chang and Jeong, {Seok Hoon}",

note = "Funding Information: This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (2011-0013018). ",

year = "2013",

month = oct,

doi = "10.1016/j.diagmicrobio.2013.06.023",

language = "English",

volume = "77",

pages = "143--149",

journal = "Diagnostic Microbiology and Infectious Disease",

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AU - Kim, Song Yee

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AU - Bae, Il Kwon

AU - Yim, Jae Joon

AU - Park, Moo Suk

AU - Kim, Young Sam

AU - Kim, Se Kyu

AU - Chang, Joon

AU - Jeong, Seok Hoon

N1 - Funding Information: This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (2011-0013018).

PY - 2013/10

Y1 - 2013/10

N2 - This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.

AB - This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.

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Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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