Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Song Yee Kim, Young Ae Kang, Il Kwon Bae, Jae Joon Yim, Moo Suk Park, Young Sam Kim, Se Kyu Kim, Joon Chang, Seok Hoon Jeong

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16 Citations (Scopus)


This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Issue number2
Publication statusPublished - 2013 Oct

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (2011-0013018).

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases


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