STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

Samir Ben Chaabane; Renyi Liu; Viswanathan Chinnusamy; Yerim Kwon; Joo Hyuk Park; Seo Yeon Kim; Jian Kang Zhu; Seong Wook Yang; Byeong Ha Lee

doi:10.1093/nar/gks1309

STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

Samir Ben Chaabane, Renyi Liu, Viswanathan Chinnusamy, Yerim Kwon, Joo Hyuk Park, Seo Yeon Kim, Jian Kang Zhu, Seong Wook Yang, Byeong Ha Lee

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97 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

Original language	English
Pages (from-to)	1984-1997
Number of pages	14
Journal	Nucleic acids research
Volume	41
Issue number	3
DOIs	https://doi.org/10.1093/nar/gks1309
Publication status	Published - 2013 Feb

Bibliographical note

Funding Information:
The National Research Foundation of Korea by the Korean Government MEST [2011-0027376 to B.-h.L., 2012R1A1A2008826 to B.-h.L.]; Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009104 to B.-h.L.]; Sogang University Research [201214003.01 to B.-h.L.]; UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274 to S.W.Y.]; Chinese Academy of Sciences and National Institutes of Health Grants [R01GM070795 to J.-K.Z., R01GM059138 to J.-K.Z.]; USDA Grant [2008-35100-04518 to J.-K.Z.]; University of California at Riverside Initial Complement Fund and a United States Department of Agriculture hatch fund [CA-R-BPS-7754H to R.L.]. Funding for open access charge: National Research Foundation of Korea by the Korean Government MEST [2012R1A1A2008826 to B.-h.L.]

Funding Information:
The authors thank laboratory members for helpful discussion and Brian Christopher King for helpful comments on the manuscript. S.W.Y. gratefully acknowledges support from Center for Synthetic Biology at University of Copenhagen and experimental equipment support from iNtRON Biotechnology, Inc. Korea.

All Science Journal Classification (ASJC) codes

Genetics

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10.1093/nar/gks1309

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@article{02526726891e4ae282b95da049160fec,

title = "STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis",

abstract = "MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.",

author = "{Ben Chaabane}, Samir and Renyi Liu and Viswanathan Chinnusamy and Yerim Kwon and Park, {Joo Hyuk} and Kim, {Seo Yeon} and Zhu, {Jian Kang} and Yang, {Seong Wook} and Lee, {Byeong Ha}",

note = "Funding Information: The National Research Foundation of Korea by the Korean Government MEST [2011-0027376 to B.-h.L., 2012R1A1A2008826 to B.-h.L.]; Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009104 to B.-h.L.]; Sogang University Research [201214003.01 to B.-h.L.]; UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274 to S.W.Y.]; Chinese Academy of Sciences and National Institutes of Health Grants [R01GM070795 to J.-K.Z., R01GM059138 to J.-K.Z.]; USDA Grant [2008-35100-04518 to J.-K.Z.]; University of California at Riverside Initial Complement Fund and a United States Department of Agriculture hatch fund [CA-R-BPS-7754H to R.L.]. Funding for open access charge: National Research Foundation of Korea by the Korean Government MEST [2012R1A1A2008826 to B.-h.L.] Funding Information: The authors thank laboratory members for helpful discussion and Brian Christopher King for helpful comments on the manuscript. S.W.Y. gratefully acknowledges support from Center for Synthetic Biology at University of Copenhagen and experimental equipment support from iNtRON Biotechnology, Inc. Korea.",

year = "2013",

month = feb,

doi = "10.1093/nar/gks1309",

language = "English",

volume = "41",

pages = "1984--1997",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

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TY - JOUR

T1 - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

AU - Ben Chaabane, Samir

AU - Liu, Renyi

AU - Chinnusamy, Viswanathan

AU - Kwon, Yerim

AU - Park, Joo Hyuk

AU - Kim, Seo Yeon

AU - Zhu, Jian Kang

AU - Yang, Seong Wook

AU - Lee, Byeong Ha

N1 - Funding Information: The National Research Foundation of Korea by the Korean Government MEST [2011-0027376 to B.-h.L., 2012R1A1A2008826 to B.-h.L.]; Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009104 to B.-h.L.]; Sogang University Research [201214003.01 to B.-h.L.]; UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274 to S.W.Y.]; Chinese Academy of Sciences and National Institutes of Health Grants [R01GM070795 to J.-K.Z., R01GM059138 to J.-K.Z.]; USDA Grant [2008-35100-04518 to J.-K.Z.]; University of California at Riverside Initial Complement Fund and a United States Department of Agriculture hatch fund [CA-R-BPS-7754H to R.L.]. Funding for open access charge: National Research Foundation of Korea by the Korean Government MEST [2012R1A1A2008826 to B.-h.L.] Funding Information: The authors thank laboratory members for helpful discussion and Brian Christopher King for helpful comments on the manuscript. S.W.Y. gratefully acknowledges support from Center for Synthetic Biology at University of Copenhagen and experimental equipment support from iNtRON Biotechnology, Inc. Korea.

PY - 2013/2

Y1 - 2013/2

N2 - MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

AB - MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processingof primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesisdefective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

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SP - 1984

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JO - Nucleic acids research

JF - Nucleic acids research

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ER -

STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis

Abstract

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