TY - GEN
T1 - Spheroid cell culture on PDMS hydrophobic surfaces and integration into microfludic devices
AU - Ingram, Patrick
AU - Im, Maesoon
AU - McDermott, Sean
AU - Wicha, Max
AU - Yoon, Euisik
PY - 2011
Y1 - 2011
N2 - We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triangular, square, and honeycomb), but only the honeycomb hydrophobic design was capable of maintaining hydrophobicity to prevent cell adhesion. With the honeycomb hydrophobic surface, cancer spheroids were grown in suspension starting from single cancer stem cells both in macro-scale wells and in integrated microfluidic microwells.
AB - We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triangular, square, and honeycomb), but only the honeycomb hydrophobic design was capable of maintaining hydrophobicity to prevent cell adhesion. With the honeycomb hydrophobic surface, cancer spheroids were grown in suspension starting from single cancer stem cells both in macro-scale wells and in integrated microfluidic microwells.
UR - http://www.scopus.com/inward/record.url?scp=84883802998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883802998&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883802998
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1539
EP - 1541
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -
